Active Recombinant Human DNMT3B, GST-tagged

• Cat.No.: DNMT3B-165H
• Product Overview: Recombinant full-length human DNMT3B was expressed by baculovirus in Sf9 insect cells using an N-terminal GST tag.

Specification

• Description: DNMT3B or DNA (cytosine-5-)-methyltransferase 3 beta encodes a DNA methyltransferase which is thought to function in de novo methylation, rather than maintenance methylation. DNMT3B can methylate unmethylated and hemimethylated DNA with equal efficiencies. DNMT3B localizes primarily to the nucleus and its expression is developmentally regulated and is required for genome wide de novo methylation and is essential for mammalian development. Mutations in DNMT3B casue the immunodeficiency-centromeric instability-facial anomalies (ICF) syndrome.
• Source: Sf9 insect cells using baculovirus
• Species: Human
• Tag: GST
• Form: Recombinant protein stored in 50mM Tris-HCl, pH 7.5, 50mM NaCl, 10mM glutathione, 0.1mM EDTA, 0.25mM DTT, 0.1mM PMSF, 25% glycerol.
• Bio-activity: The specific activity of DNMT3B was determined to be 525 pmol /min/mg
• Molecular Mass: ~138 kDa
• Purity: >75% by densitometry
• Applications: Methyltransferase Assay
• Storage: Store product at –70 centigrade. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.
• Concentration: 0.1 μg/μl

Gene Information

• Gene Name: DNMT3B DNA (cytosine-5-)-methyltransferase 3 beta [ Homo sapiens ]
• Official Symbol: DNMT3B
• Synonyms: DNMT3B; DNA (cytosine-5-)-methyltransferase 3 beta; DNA (cytosine-5)-methyltransferase 3B; DNA MTase HsaIIIB; DNA methyltransferase HsaIIIB; ICF; ICF1; M.HsaIIIB
• Gene ID: 1789
• mRNA Refseq: NM_001207055
• Protein Refseq: NP_001193984
• MIM: 602900
• UniProt ID: Q9UBC3
• Chromosome Location: 20q11.2
• Pathway: Cysteine and methionine metabolism, organism-specific biosystem; Cysteine and methionine metabolism, conserved biosystem; Metabolic pathways, organism-specific biosystem; Methionine degradation, organism-specific biosystem; Methionine degradation, conserved biosystem; One Carbon Metabolism, organism-specific biosystem
• Function: DNA (cytosine-5-)-methyltransferase activity; DNA (cytosine-5-)-methyltransferase activity; DNA (cytosine-5-)-methyltransferase activity; DNA (cytosine-5-)-methyltransferase activity, acting on CpG substrates; DNA binding; DNA-methyltransferase activity; metal ion binding; protein binding; transcription corepressor activity; transcription corepressor activity; transferase activity; unmethylated CpG binding

Reviews

11/09/2020

The DNMT3B protein is of exceptional quality and has proven to be an ideal choice to meet my experimental requirements.

09/10/2020

With its consistent and outstanding results, the DNMT3B protein is an indispensable tool that I highly recommend for researchers in need of reliable and precise protein analysis.

04/23/2019

Its purity and reliability have consistently resulted in successful outcomes in my research projects, providing me with accurate and reproducible data.

Q&As

What are the potential side effects of targeting DNMT3B for therapeutic purposes?

Targeting DNMT3B for therapeutic purposes may lead to off-target effects and unintended changes in DNA methylation, potentially causing adverse effects on normal cellular function and gene expression.

Are there any genetic disorders associated with mutations in the DNMT3B gene?

Yes, mutations in the DNMT3B gene are linked to a rare genetic disorder called ICF (Immunodeficiency, Centromeric instability, Facial anomalies) syndrome, characterized by immune system deficiencies, facial abnormalities, and unstable centromeric regions in chromosomes.

Can DNMT3B protein be used as a potential biomarker for cancer diagnosis or prognosis?

Research has shown that alterations in DNMT3B expression or activity can be associated with various types of cancer, making it a potential biomarker for cancer diagnosis, prognosis, and treatment response.

How does DNMT3B protein contribute to epigenetic regulation in cells?

DNMT3B plays a crucial role in establishing de novo DNA methylation patterns during embryonic development and cell differentiation, contributing to the epigenetic regulation of gene expression.

What are the therapeutic implications of targeting DNMT3B in cancer treatment?

Inhibition of DNMT3B activity through targeted therapy or epigenetic modifying agents has been explored as a potential treatment strategy to reverse abnormal DNA methylation patterns and restore normal gene expression in cancer cells.