Active Recombinant Human DOT1L protein, GST-tagged

• Cat.No.: DOT1L-180H
• Product Overview: Recombinant Human DOT1L(1-435 aa) fused with GST tag at N-terminal was expressed in Insect cells.

Specification

• Description: DOT1-like protein (DOT1L) specifically methylates lysine-79 of histone H3 in the globular domain. DOT1L lacks a SET-domain, which is commonly considered to mediate lysine methylation of histones. DOT1 homologs exist in a variety of eukaryotic organisms. The activity of this methyltransferase is involved in cell cycle regulation and leukemogenesis.
• Source: Insect cells
• Species: Human
• Tag: GST
• Form: 50mM Tris-HCl, pH 7.5, 150mM NaCl, 10mM glutathione, 0.1mM EDTA, 0.25mM DTT, 0.1mM PMSF, 25% glycerol.
• Bio-activity: 14 nmol/min/mg
• Molecular Mass: 78 kDa
• Protein length: 1-435 aa
• Purity: >90%
• Applications: Methyltransferase Assay
• Stability: 1 year at -70 centigrade from the date of shipment
• Storage: Store product at –70 centigrade. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.
• Concentration: 0.1μg/μl

Gene Information

• Gene Name: DOT1L DOT1-like, histone H3 methyltransferase (S. cerevisiae) [ Homo sapiens ]
• Official Symbol: DOT1L
• Synonyms: DOT1L; DOT1-like, histone H3 methyltransferase (S. cerevisiae); histone-lysine N-methyltransferase, H3 lysine-79 specific; DOT1; histone methyltransferase DOT1L; KIAA1814; KMT4; H3-K79-HMTase; DOT1-like protein; lysine N-methyltransferase 4; histone H3-K7
• Gene ID: 84444
• mRNA Refseq: NM_032482
• Protein Refseq: NP_115871
• MIM: 607375
• UniProt ID: Q8TEK3
• Chromosome Location: 19p13.3
• Pathway: Lysine degradation, organism-specific biosystem; Lysine degradation, conserved biosystem; Transcriptional misregulation in cancer, organism-specific biosystem; Transcriptional misregulation in cancer, conserved biosystem
• Function: DNA binding; histone-lysine N-methyltransferase activity; methyltransferase activity; protein binding; transcription factor binding; transferase activity

Reviews

11/04/2022

Efficient protein purification, consistently delivers high yield and purity.

11/21/2020

Precise protein quantification, ensures data precision in our experiments.

02/11/2018

Dependable peptide synthesis, accelerates our peptide-focused projects effectively.

Q&As

What are the mechanisms by which DOT1L modulates transcriptional regulation in various cell types?

DOT1L modulates transcriptional regulation, affecting gene expression patterns in different cell types.

How do alterations in DOT1L expression or activity affect cellular aging and the development of age-related diseases?

Variations in DOT1L activity impact cellular aging and may contribute to age-related diseases.

How does DOT1L influence embryonic development and stem cell differentiation?

DOT1L influences embryonic development and stem cell differentiation through epigenetic regulation.

How does DOT1L contribute to the pathogenesis of leukemia, particularly MLL-rearranged leukemia?

DOT1L contributes to leukemia development, especially in MLL-rearranged cases, by altering gene expression.

How does DOT1L (Disruptor of Telomeric Silencing 1-Like) regulate histone methylation and gene expression?

DOT1L regulates gene expression by methylating histone H3, affecting chromatin and gene activity.

What role does DOT1L play in the development and maintenance of chromatin structure?

DOT1L plays a crucial role in chromatin structure and stability, influencing genomic integrity.

What is the impact of DOT1L inhibition on epigenetic therapies for cancer treatment?

Inhibiting DOT1L shows promise in epigenetic cancer therapies by modifying disease-related gene expression.