Active Recombinant Human DSG2 protein, His-tagged

• Cat.No.: DSG2-510H
• Product Overview: Recombinant Human DSG2 protein(Q14126)(Ala50-Gly609) is expressed from HEK293 with His tag at the C-terminal.

Specification

• Source: HEK293
• Species: Human
• Tag: His
• Form: Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
• Bio-activity: Immobilized Human DSG-2, His Tag at 0.5μg/ml (100μl/well) on the plate. Dose response curve for Anti-DSG-2 Antibody, hFc Tag with the EC50 of 10.7ng/ml determined by ELISA.
• Molecular Mass: The protein has a predicted MW of 63.6 kDa. Due to glycosylation, the protein migrates to 75-80 kDa based on Tris-Bis PAGE result.
• Protein Length: Ala50-Gly609
• Endotoxin: Less than 1EU per μg by the LAL method.
• Purity: > 95% as determined by Tris-Bis PAGE
  > 95% as determined by HPLC
• Storage: -20 to -80°C for 12 months as supplied from date of receipt.
  -20 to -80°C for 3-6 months in unopened state after reconstitution.
  2-8°C for 2-7 days after reconstitution.
  Recommend to aliquot the protein into smaller quantities for optimal storage. Please avoid freeze-thaw cycles.
• Reconstitution: Centrifuge tubes before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.

Gene Information

• Gene Name: DSG2 desmoglein 2 [ Homo sapiens ]
• Official Symbol: DSG2
• Synonyms: DSG2; desmoglein 2; desmoglein-2; CDHF5; HDGC; cadherin family member 5; ARVC10; ARVD10; CMD1BB; MGC117034; MGC117036; MGC117037
• Gene ID: 1829
• mRNA Refseq: NM_001943
• Protein Refseq: NP_001934
• MIM: 125671
• UniProt ID: Q14126

Reviews

05/05/2021

Unmatched product superiority, professional customer service, and delivery that exceeded expectations.

02/01/2019

A truly delightful buying experience.

07/17/2018

This lab product excels in quality, backed by a customer service team that is not only friendly but also knowledgeable.

Q&As

How does Desmoglein-2 contribute to wound healing and tissue repair, and are there therapeutic implications for manipulating its activity in these processes?

Desmoglein-2 plays a role in wound healing and tissue repair by facilitating cell adhesion and migration. Manipulating its activity may have therapeutic implications for enhancing these processes.

How is the expression of Desmoglein-2 regulated, and what are the consequences of its dysregulation in the context of autoimmune diseases and skin disorders?

Desmoglein-2 expression is regulated at transcriptional and post-translational levels. Dysregulation is linked to autoimmune diseases like pemphigus, where autoantibodies target Desmoglein-2, leading to skin blistering.

Can you elaborate on the molecular structure of Desmoglein-2 and its interactions within desmosomal complexes?

Desmoglein-2 has a unique molecular structure with extracellular domains facilitating cell-cell adhesion. It interacts with other desmosomal components, such as Desmocollins and Plakoglobin, forming strong intercellular junctions.

How does Desmoglein-2 contribute to the regulation of cell signaling pathways, and are there specific pathways influenced by its interactions with other cellular components?

Desmoglein-2 participates in cell signaling, impacting pathways involved in cell adhesion, migration, and survival. Its interactions with other desmosomal components modulate signaling cascades essential for cellular function.

Can you discuss the role of Desmoglein-2 in epithelial-mesenchymal transition (EMT) and its implications for cancer invasion and metastasis?

Desmoglein-2 influences EMT, a process crucial for cancer invasion and metastasis. Changes in Desmoglein-2 expression and localization contribute to alterations in cell adhesion dynamics during EMT.