Active Recombinant Human EEF2K(2-end) Protein, N-GST-tagged

• Cat.No.: EEF2K-58H
• Product Overview: N-terminal GSTtagged, recombinant, human eEF-2K, amino acids 2–end, expressed in E. coli cells. Purified using glutathione agarose.

Specification

• Description: This gene encodes a highly conserved protein kinase in the calmodulin-mediated signaling pathway that links activation of cell surface receptors to cell division. This kinase is involved in the regulation of protein synthesis. It phosphorylates eukaryotic elongation factor 2 (EEF2) and thus inhibits the EEF2 function. The activity of this kinase is increased in many cancers and may be a valid target for anti-cancer treatment.
• Source: E. coli
• Species: Human
• Tag: GST
• Protein length: 2-end
• Form: 1.805mg/ml of enzyme in 50mM Tris/HCl pH7.5, 150mM NaCl, 0.1mM EGTA, 0.03% Brij-35, 270mM sucrose, 1mM benzamidine, 0.2mM PMSF, 0.1% 2-mercaptoethanol. Frozen solution.
• Bio-activity: 1050U/mg, where one unit of eEF-2K,a ctive activity is defined as 1nmol phosphate incorporated into 300μM (RKKFGESEKTKTKEFL) per minute at 30 centigrade with a final ATP concentration of 100μM.
• Molecular Mass: 108.5 kDa
• AA Sequence: 1 MSPILGYWKI KGLVQPTRLL LEYLEEKYEE HLYERDEGDK WRNKKFELGL EFPNLPYYID 61 GDVKLTQSMA IIRYIADKHN MLGGCPKERA EISMLEGAVL DIRYGVSRIA YSKDFETLKV 121 DFLSKLPEML KMFEDRLCHK TYLNGDHVTH PDFMLYDALD VVLYMDPMCL DAFPKLVCFK 181 KRIEAIPQID KYLKSSKYIA WPLQGWQATF GGGDHPPKSD LVPRGSADED LIFRLEGVDG 241 GQSPRAGRDG DSDGDSDDEE GYFICPITDD PSSNQNVNSK VNKYYSNLTK SERYSSSGSP 301 ANSFHFKEAW KHAIQKAKHM PDPWAEFHLE DIATERATRH RYNAVTGEWL DDEVLIKMAS 361 QPFGRGAMRE CFRTKKLSNF LHAQQWKGAS NYVAKRYIEP VDRDVYFEDV RLQMEAKLWG 421 EEYNRHKPPK QVDIMQMCII ELKDRPGKPL FHLEHYIEGK YIKYNSNSGF VRDDNIRLTP 481 QAFSHFTFER SGHQLIVVDI QGVGDLYTDP QIHTETGTDF GDGNLGVRGM ALFFYSHACN 541 RICESMGLAP FDLSPRERDA VNQNTKLLQS AKTILRGTEE KCGSPRVRTL SGSRPPLLRP 601 LSENSGDENM SDVTFDSLPS SPSSATPHSQ KLDHLHWPVF SDLDNMASRD HDHLDNHRES 661 ENSGDSGYPS EKRGELDDPE PREHGHSYSN RKYESDEDSL GSSGRVCVEK WNLLNSSRLH 721 LPRASAVALE VQRLNALDLE KKIGKSILGK VHLAMVRYHE GGRFCEKGEE WDQESAVFHL 781 EHAANLGELE AIVGLGLMYS QLPHHILADV SLKETEENKT KGFDYLLKAA EAGDRQSMIL 841 VARAFDSGQN LSPDRCQDWL EALHWYNTAL EMTDCDEGGE YDGMQDEPRY MMLAREAEML 901 FTGGYGLEKD PQRSGDLYTQ AAEAAMEAMK GRLANQYYQK AEEAWAQMEE
• Purity: Purity 83.4% by SDS-PAGE and Coomassie blue staining.
• Applications: Drug Discovery & Development
• Storage: 6 months at -70 centigrade.
• Handling Advice: Rapidly thaw the vial under cold water and immediately place on ice. Aliquot unused material into prechilled micro-centrifuge tubes and immediately snap-freeze the vials in liquid nitrogen prior to re-storage at -70 centigrade.
• Use/Stability: On receipt of material store at -70 centigrade. Unopened reagent is stable for a minimum of 1 year from date of shipment when stored at recommended storage temperature. Avoid repeat freeze/thaw cycles. For maximum recovery of product, centrifuge original vial prior to removing the cap.

Gene Information

• Gene Name: EEF2K eukaryotic elongation factor 2 kinase [ Homo sapiens (human) ]
• Official Symbol: EEF2K
• Synonyms: EEF2K; eukaryotic elongation factor 2 kinase; eEF-2K; CaMKIII; HSU93850
• Gene ID: 29904
• mRNA Refseq: NM_013302.5
• Protein Refseq: NP_037434.2
• MIM: 606968
• UniProt ID: O00418