| Description : |
Epidermal Growth Factor (EGF) is a polypeptide growth factor which stimulates the proliferation of a wide range of epidermal and epithelial cells. |
| Source : |
E. coli |
| Species : |
Human |
| Form : |
Sterile Filtered White lyophilized (freeze-dried) powder. |
| Bio-activity : |
The ED50, calculated by the dose-dependant proliferation of murine BALB/c 3T3 cells is less then 2 ng/mL, corresponding to a specific activity of 5.0 × 10^5 IU/mg. |
| Molecular Mass : |
6.0 kDa+/-10% determined by reduced SDS-PAGE |
| Protein Length : |
53 |
| AA Sequence : |
MNSDSECPLSHDGYCLHDGVCMYIE ALDKYACNCVVGYIGERCQYRDLKW WELR |
| Endotoxin : |
Less than 0.1 ng/μg (1 EU/μg) of recombinant human Epidermal Growth Factor (EGF) as determined by LAL test. |
| Purity : |
Greater than 95% as determined by (a) Analysis by SEC-HPLC (b) Analysis by reducing and non-reducing SDS-PAGE Silver Stained gel |
| Storage : |
Lyophilized recombinant Human Epidermal Growth Factor (rhEGF) remains stable up to 12 months at -80 centigrade from date of receipt. Upon reconstitution, rhEGF should be stable up to 4 weeks at 4 centigrade or up to 6 months at -20 centigrade. |
| Storage Buffer : |
Recombinant human Epidermal Growth Factor (EGF) was lyophilized after extensive dialysis against 10mM Phosphate buffer, pH7.0, 200mM NaCl buffer. |
| Reconstitution : |
It is recommended to reconstitute the lyophilized recombinant human Epidermal Growth Factor (EGF) in sterile 18 MΩ-cm H2O not less than 100 μg/mL, which can then be further diluted to other aqueous solutions. |
