Active Recombinant Human EGFR Protein (Leu 25 - Ser 645), Fc-tagged
Cat.No. : | EGFR-339H |
Product Overview : | Recombinant Human EGFR Protein, Fc-tagged is expressed from human 293 cells (HEK293). It contains AA Leu 25 - Ser 645 (Accession # NP_005219). Predicted N-terminus: Leu 25. This protein carries a human IgG1 Fc tag at the C-terminus. The protein has a calculated MW of 95 kDa. The protein migrates as 130-140 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation. |
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Source : | HEK293 |
Species : | Human |
Tag : | Fc |
Form : | Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization. |
Bio-activity : | Immobilized Human EGF, Tag Free at 10μg/mL (100 μL/well) can bind Recombinant Human EGFR Protein, Fc-tagged with a linear range of 0.2-3.2 μg/mL (QC tested). Recombinant Human EGFR Protein, Fc-tagged at 1 μg/mL (100 μL/well) can bind Cetuximab with a linear range of 0.1-2 ng/mL (Routinely tested). |
Molecular Mass : | 95 kDa |
Protein length : | Leu 25 - Ser 645 |
Endotoxin : | Less than 1.0 EU per μg by the LAL method. |
Purity : | >95% as determined by SDS-PAGE. |
Notes : | Please avoid repeated freeze-thaw cycles. |
Stability : | No activity loss is observed after storage at: 4-8 centigrade for 12 months in lyophilized state; -70 centigrade for 3 months under sterile conditions after reconstitution. |
Storage : | For long term storage, the product should be stored at lyophilized state at -20 centigrade or lower. |
Reconstitution : | It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 mg/ml. Centrifuge the vial at 4 centigrade before opening to recover the entire contents. |
Gene Name : | EGFR epidermal growth factor receptor [ Homo sapiens ] |
Official Symbol : | EGFR |
Synonyms : | EGFR; epidermal growth factor receptor; ERBB1; ERBB; HER1; mENA; PIG61; |
Gene ID : | 1956 |
mRNA Refseq : | NM_005228 |
Protein Refseq : | NP_005219 |
MIM : | 131550 |
UniProt ID : | P00533 |
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For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.
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