Active Recombinant Human EGFR Protein (Leu 25 - Ser 645), His-tagged, low endotoxin

• Cat.No.: EGFR-338H
• Product Overview: Recombinant Human EGFR Protein, His-tagged, low endotoxin is expressed from human 293 cells (HEK293). It contains AA Leu 25 - Ser 645 (Accession # P00533-1). Predicted N-terminus: Leu 25. This protein carries a polyhistidine tag at the C-terminus. The protein has a calculated MW of 70.5kDa. The protein migrates as 110-115 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

Specification

• Source: HEK293
• Species: Human
• Tag: His
• Form: Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization.
• Bio-activity: Immobilized Cetuximab at 2 μg/mL (100 μL/well) can bind Recombinant Human EGFR Protein, His-tagged, low endotoxin with a linear range of 0.8-6 ng/mL (QC tested).
  Immobilized Erbitux on CM5 Chip via anti-human Fc IgG, can bind Recombinant Human EGFR Protein, His-tagged, low endotoxin with an affinity constant of 0.492 nM as determined in SPR assay (Biacore T200) (Routinely tested).
• Molecular Mass: 70.5kDa
• Protein length: Leu 25 - Ser 645
• Endotoxin: Less than 0.01 EU per μg by the LAL method.
• Purity: >95% as determined by SDS-PAGE.
• Notes: Please avoid repeated freeze-thaw cycles.
• Stability: No activity loss is observed after storage at:
  4-8 centigrade for 12 months in lyophilized state;
  -70 centigrade for 3 months under sterile conditions after reconstitution.
• Storage: For long term storage, the product should be stored at lyophilized state at -20 centigrade or lower.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 mg/ml. Centrifuge the vial at 4 centigrade before opening to recover the entire contents.

Gene Information

• Gene Name: EGFR epidermal growth factor receptor [ Homo sapiens ]
• Official Symbol: EGFR
• Synonyms: EGFR; epidermal growth factor receptor; ERBB1; ERBB; HER1; mENA; PIG61
• Gene ID: 1956
• mRNA Refseq: NM_005228
• Protein Refseq: NP_005219
• MIM: 131550
• UniProt ID: P00533