| Description : |
EGFR (Epidermal Growth Factor Receptor), also known as ErbB1 or HER1, is a transmembrane receptor protein that belongs to the family of receptor tyrosine kinases. It is involved in a variety of cellular processes, including cell growth, proliferation, differentiation, and survival. EGFR is activated by binding to specific ligands, such as epidermal growth factor (EGF) or transforming growth factor alpha (TGF-alpha), which leads to dimerization of the receptor and autophosphorylation of specific tyrosine residues on the intracellular domain. This activates downstream signaling pathways, such as the MAPK/ERK, PI3K/AKT, and JAK/STAT pathways, which regulate gene expression and other cellular functions. Aberrant activation of EGFR has been implicated in several diseases, including cancer, inflammatory disorders, and neurodegenerative diseases. |
| Source : |
Sf21 |
| Species : |
Human |
| Tag : |
GST |
| Protein length : |
696-end |
| Form : |
0.379mg/ml of enzyme in 50mM Tris/HCl pH7.5, 300mM NaCl, 0.1mM EGTA, 0.03% Brij-35, 270mM sucrose, 1mM benzamidine, 0.2mM PMSF, 0.1% 2-mercaptoethanol. Frozen solution. |
| Bio-activity : |
285U/mg, where one unit of EGFR (T790M) activity is defined as 1nmol phosphate incorporated into 250μM (GGMEDIYFEFMGGKKK) per minute at 30 centigrade with a final ATP concentration of 100μM. |
| Molecular Mass : |
86 kDa |
| AA Sequence : |
1 MSPILGYWKI KGLVQPTRLL LEYLEEKYEE HLYERDEGDK WRNKKFELGL EFPNLPYYID 61 GDVKLTQSMA IIRYIADKHN MLGGCPKERA EISMLEGAVL DIRYGVSRIA YSKDFETLKV 121 DFLSKLPEML KMFEDRLCHK TYLNGDHVTH PDFMLYDALD VVLYMDPMCL DAFPKLVCFK 181 KRIEAIPQID KYLKSSKYIA WPLQGWQATF GGGDHPPKSD LEVLFQGPEF KGMGIRNSKG 241 GEAPNQALLR ILKETEFKKI KVLGSGAFGT VYKGLWIPEG EKVKIPVAIK ELREATSPKA 301 NKEILDEAYV MASVDNPHVC RLLGICLTST VQLIMQLMPF GCLLDYVREH KDNIGSQYLL 361 NWCVQIAKGM NYLEDRRLVH RDLAARNVLV KTPQHVKITD FGLAKLLGAE EKEYHAEGGK 421 VPIKWMALES ILHRIYTHQS DVWSYGVTVW ELMTFGSKPY DGIPASEISS ILEKGERLPQ 481 PPICTIDVYM IMVKCWMIDA DSRPKFRELI IEFSKMARDP QRYLVIQGDE RMHLPSPTDS 541 NFYRALMDEE DMDDVVDADE YLIPQQGFFS SPSTSRTPLL SSLSATSNNS TVACIDRNGL 601 QSCPIKEDSF LQRYSSDPTG ALTEDSIDDT FLPVPEYINQ SVPKRPAGSV QNPVYHNQPL 661 NPAPSRDPHY QDPHSTAVGN PEYLNTVQPT CVNSTFDSPA HWAQKGSHQI SLDNPDYQQD 721 FFPKEAKPNG IFKGSTAENA EYLRVAPQSS EFIGA |
| Purity : |
Purity 85.2% by SDS-PAGE and Coomassie blue staining. |
| Applications : |
Drug Discovery & Development |
| Storage : |
6 months at -70 centigrade. |
| Handling Advice : |
Rapidly thaw the vial under cold water and immediately place on ice. Aliquot unused material into prechilled micro-centrifuge tubes and immediately snap-freeze the vials in liquid nitrogen prior to re-storage at -70 centigrade. |
| Use/Stability : |
On receipt of material store at -70 centigrade. Unopened reagent is stable for a minimum of 1 year from date of shipment when stored at recommended storage temperature. Avoid repeat freeze/thaw cycles. For maximum recovery of product, centrifuge original vial prior to removing the cap. |
