Active Recombinant Human EPCAM Protein, Fc-tagged, Alexa Fluor 555 conjugated
Cat.No. : | EPCAM-475HAF555 |
Product Overview : | Alexa Fluor 555 conjugated recombinant human EPCAM(Gln24 - Lys265) fused with Fc tag at C-terminal was expressed in NS0. |
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Source : | Mammalian cells |
Species : | Human |
Tag : | Fc |
Form : | Lyophilized |
Bio-activity : | Measured by the ability of the immobilized protein to support the adhesion of the L Cells mouse fibroblast cell line.When 5 × 10^4 cells/well are added to recombinant human EpCAM/Fc Chimera and human Fibronectin (0.5 μg/mL) coated plates, cell adhesion is enhanced in a dose dependent manner after 45 minutes at 37 centigrade. The ED50 for this effect is typically 0.7-2.8 μg/mL. Measured by its binding ability in a functional ELISA. The activity of this protein is measured in a homophilic binding assay. |
Molecular Mass : | Predicted Molecular Mass: 54 (monomer) kDa; SDS-PAGE: 60-70 kDa, reducing conditions |
Protein length : | Gln24-Lys265 |
N-terminal Sequence Analysis : | Gln 24 |
Endotoxin : | < 0.1 EU/ μg of the protein by the LAL method. |
Purity : | > 90 % by SDS-PAGE and analyzed by silver stain |
Characteristic : | Disulfide-linked homodimer Labeled with Alexa Fluor 555 via amines With an excitation and emission maximum of 555/565 nm, Alexa Fluor 555 can be efficiently excited using a 543 nm He-Ne laser line and detected under standard TRITC/Cy3 filters. |
Storage : | Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 centigrade as supplied. 1 month, 2 to 8 centigrade under sterile conditions after reconstitution. 3 months, -20 to -70 centigrade under sterile conditions after reconstitution. |
Concentration : | 100 μg/mL |
Storage Buffer : | Lyophilized from a 0.2 μm filtered solution in PBS. |
Reconstitution : | Reconstitute at 100 μg/mL in sterile PBS. |
Shipping : | The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. |
Gene Name : | EPCAM epithelial cell adhesion molecule [ Homo sapiens ] |
Official Symbol : | EPCAM |
Synonyms : | EPCAM; epithelial cell adhesion molecule; antigen identified by monoclonal AUA1 , M4S1, MIC18, TACSTD1, tumor associated calcium signal transducer 1; 17 1A; 323/A3; CD326; CO 17A; EGP 2; EGP34; EGP40; Ep CAM; ESA; GA733 2; HEA125; KS1/4; KSA; Ly74; MH99; MK 1; MOC31; TACST 1; TROP1; epithelial glycoprotein 314; human epithelial glycoprotein-2; cell surface glycoprotein Trop-1; adenocarcinoma-associated antigen; tumor-associated calcium signal transducer 1; major gastrointestinal tumor-associated protein GA733-2; membrane component, chromosome 4, surface marker (35kD glycoprotein); M4S1; MK-1; DIAR5; EGP-2; MIC18; EGP314; HNPCC8; TACSTD1; GA733-2; |
Gene ID : | 4072 |
mRNA Refseq : | NM_002354 |
Protein Refseq : | NP_002345 |
MIM : | 185535 |
UniProt ID : | P16422 |
Products Types
◆ Recombinant Protein | ||
EPCAM-2666H | Recombinant Human EPCAM protein(161-250 aa), C-His-tagged | +Inquiry |
EPCAM-215H | Active Recombinant Human EPCAM Protein (Gln24-Lys265), C-His tagged, Animal-free, Carrier-free | +Inquiry |
EPCAM-097H | Recombinant Human EPCAM Protein, His-tagged | +Inquiry |
EPCAM-1430H | Recombinant Human EPCAM Protein, His&GST-tagged | +Inquiry |
EPCAM-349H | Recombinant Human EPCAM Protein, Strep-tagged | +Inquiry |
◆ Lysates | ||
EPCAM-2143MCL | Recombinant Mouse EPCAM cell lysate | +Inquiry |
EPCAM-001CCL | Recombinant Cynomolgus EPCAM cell lysate | +Inquiry |
EPCAM-2525HCL | Recombinant Human EPCAM cell lysate | +Inquiry |
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For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.
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