| Description : |
Eph family tyrosine kinase receptors consist of two groups: EphA group members respond to ephrin-A, which is anchored to the cell membrane by glycosylphosphatidylinositol, whereas EphB group members respond to ephrin-B, containing a transmembrane domain. Among them, only EPA4 (EphA4, Ephrin type-A receptor 4, Tyrosine-protein kinase receptor SEK, Receptor protein-tyrosine kinase HEK8) can cross-respond to both ephrin-A and -B. In the final stages of embryogenesis, expression of EphA4 is found predominantly within regions of the central nervous system including the cerebral cortex, striatum, thalamus, hippocampus, and ventral spinal cord. EphA4 null mutant mice showed specific defects in the corticospinal tract. Recently, the Eph-ephrin system has been found to be involved in vascular development, angiogenesis, tumor growth and metastasis as well as regulation of smooth muscle contractility, onset of platelet aggregation and to promote onset, growth and stability of the hemostatic plugs. Inhibition of EPA4 may be used in the treatment of various tumors but may delay wound healing. |
| Source : |
Sf21 |
| Species : |
Human |
| Tag : |
His |
| Protein length : |
601-892 aa |
| Form : |
1.939mg/ml of enzyme in 50mM Tris/HCl pH8.0, 300mM NaCl, 0.1mM EGTA, 0.03% Brij-35, 270mM sucrose, 1mM benzamidine, 0.2mM PMSF, 0.1% 2-mercaptoethanol. Frozen solution. |
| Bio-activity : |
136U/mg, where one unit of EphA4 activity is defined as 1nmol phosphate incorporated into 0.1mg/ml poly(Glu, Tyr) (4:1) per minute at 30 centigrade with a final ATP concentration of 100μM |
| Molecular Mass : |
37 kDa |
| AA Sequence : |
1 MSYYHHHHHH DYDIPTTENL YFQGAMSLPR TYEDPNQAVR EFAKEIDASC IKIEKVIGVG 61 EFGEVCSGRL KVPGKREICV AIKTLKAGYT DKQRRDFLSE ASIMGQFDHP NIIHLEGVVT 121 KCKPVMIITE YMENGSLDAF LRKNDGRFTV IQLVGMLRGI GSGMKYLSDM SYVHRDLAAR 181 NILVNSNLVC KVSDFGMSRV LEDDPEAAYT TRGGKIPIRW TAPEAIAYRK FTSASDVWSY 241 GIVMWEVMSY GERPYWDMSN QDVIKAIEEG YRLPPPMDCP IALHQLMLDC WQKERSDRPK 301 FGQIVNMLDK LIRNPNSLKR TG |
| Purity : |
Purity 97% by SDS-PAGE and Coomassie blue staining. |
| Applications : |
Drug Discovery & Development |
| Storage : |
6 months at -70 centigrade. |
| Handling Advice : |
Rapidly thaw the vial under cold water and immediately place on ice. Aliquot unused material into prechilled micro-centrifuge tubes and immediately snap-freeze the vials in liquid nitrogen prior to re-storage at -70 centigrade. |
| Use/Stability : |
On receipt of material store at -70 centigrade. Unopened reagent is stable for a minimum of 1 year from date of shipment when stored at recommended storage temperature. Avoid repeat freeze/thaw cycles. For maximum recovery of product, centrifuge original vial prior to removing the cap. |
