| Description : |
Eph (erythropoietin-producing hepatocellular) family tyrosine kinase receptors consist of two groups: EphA group members respond to ephrin-A, which is anchored to the cell membrane by glycosylphosphatidylinositol, whereas EphB group members respond to ephrin-B, containing a transmembrane domain. Among them, only EphA4 can cross-respond to both ephrin-A and -B. EphB1 (EPHT2, NET, Hek6, ELK) is expressed predominantly in developing neural structures in embryos, in vascular epithelium of kidney and other tissues. EphB1 signals regulation of cell attachment and cell-cell assembly and are implicated in neuronal and vascular cell targeting. |
| Source : |
Sf21 |
| Species : |
Human |
| Tag : |
His |
| Protein length : |
564-end |
| Form : |
2.069mg/ml of enzyme in 50mM Tris/HCl pH7.5, 300mM NaCl, 0.1mM EGTA, 0.03% Brij-35, 270mM sucrose, 1mM benzamidine, 0.2mM PMSF, 0.1% 2-mercaptoethanol. Frozen solution. |
| Bio-activity : |
206U/mg, where one unit of EphB1, active activity is defined as 1nmol phosphate incorporated into 250μM cdc2 peptide per minute at 30 centigrade with a final ATP concentration of 100μM. |
| Molecular Mass : |
51.4 kDa |
| AA Sequence : |
1 MSYYHHHHHH DYDIPTTENL YFQGAMDPEF SRKRAYSKEA VYSDKLQHYS TGRGSPGMKI 61 YIDPFTYEDP NEAVREFAKE IDVSFVKIEE VIGAGEFGEV YKGRLKLPGK REIYVAIKTL 121 KAGYSEKQRR DFLSEASIMG QFDHPNIIRL EGVVTKSRPV MIITEFMENG ALDSFLRQND 181 GQFTVIQLVG MLRGIAAGMK YLAEMNYVHR DLAARNILVN SNLVCKVSDF GLSRYLQDDT 241 SDPTYTSSLG GKIPVRWTAP EAIAYRKFTS ASDVWSYGIV MWEVMSFGER PYWDMSNQDV 301 INAIEQDYRL PPPMDCPAAL HQLMLDCWQK DRNSRPRFAE IVNTLDKMIR NPASLKTVAT 361 ITAVPSQPLL DRSIPDFTAF TTVDDWLSAI KMVQYRDSFL TAGFTSLQLV TQMTSEDLLR 421 IGITLAGHQK KILNSIHSMR VQISQSPTAM A |
| Purity : |
Purity 99% by SDSPAGE and Coomassie blue staining. |
| Applications : |
Drug Discovery & Development |
| Storage : |
6 months at -70 centigrade. |
| Handling Advice : |
Rapidly thaw the vial under cold water and immediately place on ice. Aliquot unused material into prechilled micro-centrifuge tubes and immediately snap-freeze the vials in liquid nitrogen prior to re-storage at -70 centigrade. |
| Use/Stability : |
On receipt of material store at -70 centigrade. Unopened reagent is stable for a minimum of 1 year from date of shipment when stored at recommended storage temperature. Avoid repeat freeze/thaw cycles. For maximum recovery of product, centrifuge original vial prior to removing the cap. |
