| Description : |
Eph family tyrosine kinase receptors consist of two groups: EphA group members respond to ephrin-A, which is anchored to the cell membrane by glycosylphosphatidylinositol, whereas EphB group members respond to ephrin-B, containing a transmembrane domain. Among them, only EphA4 can cross-respond to both ephrin-A and -B. The primary function of EphB2 (Ephrin type-B receptor 2, DRT, EPTH3, ERK, HEK5) is to inactivate the Ras-MAPK pathway in a fashion that contributes to cytoskeletal reorganization and adhesion responses in neuronal growth cones. GenBank D31661. The recombinant protein is A848S and K918E with respect to this accession number. |
| Source : |
Sf21 |
| Species : |
Human |
| Tag : |
His |
| Protein length : |
560-end |
| Form : |
2.56mg/ml of enzyme in 50mM Tris/HCl pH7.5, 150mM NaCl, 0.1mM EGTA, 0.03% Brij-35, 270mM sucrose, 0.2mM PMSF, 1mM benzamidine, 0.1% 2-mercaptoethanol. Frozen solution. |
| Bio-activity : |
153U/mg, where one unit of EphB2 activity is defined as 1nmol phosphate incorporated into 0.1mg/ml poly(Glu, Tyr) (4:1) per minute at 30 centigrade with a final ATP concentration of 100μM. |
| Molecular Mass : |
52 kDa |
| AA Sequence : |
1 MSYYHHHHHH DYDIPTTENL YFQGAMDPEF CNRRGFERAD SEYTDKLQHY TSGHMTPGMK 61 IYIDPFTYED PNEAVREFAK EIDISCVKIE QVIGAGEFGE VCSGHLKLPG KREIFVAIKT 121 LKSGYTEKQR RDFLSEASIM GQFDHPNVIH LEGVVTKSTP VMIITEFMEN GSLDSFLRQN 181 DGQFTVIQLV GMLRGIAAGM KYLADMNYVH RDLAARNILV NSNLVCKVSD FGLSRFLEDD 241 TSDPTYTSAL GGKIPIRWTA PEAIQYRKFT SASDVWSYGI VMWEVMSYGE RPYWDMTNQD 301 VINAIEQDYR LPPPMDCPSA LHQLMLDCWQ KDRNHRPKFG QIVNTLDKMI RNPNSLKAMA 361 PLSSGINLPL LDRTIPDYTS FNTVDEWLEA IKMGQYKESF ANAGFTSFDV VSQMMMEDIL 421 RVGVTLAGHQ KKILNSIQVM RAQMNQIQSV EV |
| Purity : |
Purity 79.3 % by SDS-PAGE and Coomassie blue staining. |
| Applications : |
Drug Discovery & Development |
| Storage : |
6 months at -70 centigrade. |
| Handling Advice : |
Rapidly thaw the vial under cold water and immediately place on ice. Aliquot unused material into prechilled micro-centrifuge tubes and immediately snap-freeze the vials in liquid nitrogen prior to re-storage at -70 centigrade. |
| Use/Stability : |
On receipt of material store at -70 centigrade. Unopened reagent is stable for a minimum of 1 year from date of shipment when stored at recommended storage temperature. Avoid repeat freeze/thaw cycles. For maximum recovery of product, centrifuge original vial prior to removing the cap. |
