Active Recombinant Human ERBB2(676-end) Protein, C-His-tagged

• Cat.No.: ERBB2-76H
• Product Overview: C-terminal 6His-tagged, recombinant, human ErbB2 amino acids 676- end expressed by baculovirus in Sf21 insect cells. Purified using immobilized metal affinity chromatography.

Specification

• Description: Human EGF receptor 2 (HER2, ERBB2) tyrosine kinase is an EGF receptor-related protein tyrosine kinase which is overexpressed in many mammary and ovarian carcinomas. Binding of the ligand to the receptor is believed to activate the receptor's intrinsic protein kinase activity, initiating a signal transduction pathway which leads to increased cell proliferation. Truncated HER2, lacking the extracellular and transmembrane regions, is constitutively active. The active kinase transfers g-phosphates of ATP to the phenolic hydroxyl of specific tyrosine residues on target proteins.
• Source: Sf21
• Species: Human
• Tag: His
• Protein length: 676-end
• Form: 1.871mg/ml of enzyme in 50mM Tris/HCl pH7.5, 150mM NaCl, 0.5mM EDTA, 0.01% CHAPS, 50% glycerol, 2mM DTT. Frozen solution.
• Bio-activity: 361U/mg, where one unit of ErbB2 activity is defined as 1nmol phosphate incorporated into 0.1mg/ml poly(Glu, Tyr) (4:1) per minute at 30 centigrade with a final ATP concentration of 100μM.
• Molecular Mass: 69 kDa
• AA Sequence: 1 MLVPRGSPWI RNSKAYVDKR RQQKIRKYTM RRLLQETELV EPLTPSGAMP NQAQMRILKE 61 TELRKVKVLG SGAFGTVYKG IWIPDGENVK IPVAIKVLRE NTSPKANKEI LDEAYVMAGV 121 DSPYVSRLLG ICLTSTVQLV TQLMPYGCLL DHVRENRGRL GSQDLLNWCM QIAKGMSYLE 181 DVRLVHRDLA ARNVLVKSPN HVKITDFGLA RLLDIDETEY HADGGKVPIK WMALESILRR 241 RFTHQSDVWS YGVTVWELMT FGAKPYDGIP AREIPDLLEK GERLPQPPIC TIDVYMIMVK 301 CWMIDSECRP RFRELVSEFS RMARDPQRFV VIQNEDLGPA SPLDSTFYRS LLEDDDMGDL 361 VDAEEYLVPQ QGFFCPDPAP GAGGMVHHRH RSSSTRSGGG DLTLGLEPSE EEAPRSPLAP 421 SEGAGSDVFD GDLGMGAAKG LQSLPTHDPS PLQRYSEDPT VPLPSETDGY VAPLTCSPQP 481 EYVNQPDVRP QPPSPREGPL PAARPAGATL ERPKTLSPGK NGVVKDVFAF GGAVENPEYL 541 TPQGGAAPQP HPPPAFSPAF DNLYYWDQDP PERGAPPSTF KGTPTAENPE YLGLDVPVCS 601 RHASGTDDYD IATTHHHHHH
• Purity: Purity 85% by SDS-PAGE and Coomassie blue staining.
• Applications: Drug Discovery & Development
• Storage: 6 months at -70 centigrade.
• Handling Advice: Rapidly thaw the vial under cold water and immediately place on ice. Aliquot unused material into prechilled micro-centrifuge tubes and immediately snap-freeze the vials in liquid nitrogen prior to re-storage at -70 centigrade.
• Use/Stability: On receipt of material store at -70 centigrade. Unopened reagent is stable for a minimum of 1 year from date of shipment when stored at recommended storage temperature. Avoid repeat freeze/thaw cycles. For maximum recovery of product, centrifuge original vial prior to removing the cap.

Gene Information

• Gene Name: ERBB2 erb-b2 receptor tyrosine kinase 2 [ Homo sapiens (human) ]
• Official Symbol: ERBB2
• Synonyms: ERBB2; erb-b2 receptor tyrosine kinase 2; NEU; NGL; HER2; TKR1; CD340; HER-2; VSCN2; MLN 19; MLN-19; c-ERB2; c-ERB-2; HER-2/neu; p185(erbB2)
• Gene ID: 2064
• mRNA Refseq: NM_004448.4
• Protein Refseq: NP_004439.2
• MIM: 164870
• UniProt ID: P04626