Active Recombinant Human ESR1, His-tagged

• Cat.No.: ESR1-8487H
• Product Overview: Recombinant Human ESR1, fused with His tag, was expressed in Sf21 Cells.

Specification

• Description: Several members of the nuclear receptor family are directly associated with human malignancies including breast cancer, prostate cancer and leukemia. The pathogenesis of each of these diseases is underpinned by the activities of a member of the superfamily; estrogen receptor-alpha (ER alpha) in breast cancer, androgen receptor (AR) in prostate cancer, and retinoic acid receptor alpha (RAR alpha) in acute promyelocytic leukemia . Estrogen receptors (ER) are members of the superfamily of nuclear hormone receptors whose activity is required for the normal function of the female reproductive system. Two isoforms of estrogen receptor (ER a and ER ?) have been described. They function as ligand-dependent transcriptional activators . The biological functions downstream of ER result from altered expression of direct transcriptional targets as well as secondary effects mediated by biological activities of direct targets. In the mammary gland, estrogen receptors regulate normal epithelial cell development and differentiation through their well-documented effects on transcription . Estrogens have long been known to have mitogenic functions in breast cancer cell lines and in breast tumors . Selective estrogen receptor modulatory compounds (SERMs), which bind directly to ER, can block the growth stimulatory function of estrogens.
• Source: Sf21 Cells
• Species: Human
• Tag: His
• Form: 20 mM Tris-Cl, pH 7.9, 20% Glycerol, 100 mM KCl, 1 mM DTT and 0.2 mM EDTA
• Bio-activity: 1 unit is equal to 1 ng of purified protein. 20-100 units are sufficient for an in vitro transcription assay and 100 units are sufficient for a protein-protein interaction assay.
• AA Sequence: MTMTLHTKAS GMALLHQIQG NELEPLNRPQ LKIPLERPLG EVYLDSSKPA VYNYPEGAAY EFNAAAAANA QVYGQTGLPY GPGSEAAAFG SNGLGGFPPL NSVSPSPLML LHPPPQLSPF LQPHGQQVPY YLENEPSGYT VREAGPPAFY RPNSDNRRQG GRERLASTND KGSMAMESAK ETRYCAVCND YASGYHYGVW SCEGCKAFFK RSIQGHNDYM CPATNQCTID KNRRKSCQAC RLRKCYEVGM MKGGIRKDRR GGRMLKHKRQ RDDGEGRGEV GSAGDMRAAN LWPSPLMIKR SKKNSLALSL TADQMVSALL DAEPPILYSE YDPTRPFSEA SMMGLLTNLA DRELVHMINW AKRVPGFVDL TLHDQVHLLE CAWLEILMIG LVWRSMEHPG KLLFAPNLLL DRNQGKCVEG MVEIFDMLLA TSSRFRMMNL QGEEFVCLKS IILLNSGVYT FLSSTLKSLE EKDHIHRVLD KITDTLIHLM AKAGLTLQQQ HQRLAQLLLI LSHIRHMSNK GMEHLYSMKC KNVVPLYDLL LEMLDAHRLH APTSRGGASV EETDQSHLAT AGSTSSHSLQ KYYITGEAEG FPATV
• Purity: >95% as determined by SDS-PAGE
• Storage: -80 °C
• Concentration: 200 μg/ml
• Shipping: Dry Ice

Gene Information

• Gene Name: ESR1 estrogen receptor 1 [ Homo sapiens ]
• Official Symbol: ESR1
• Synonyms: ESR1; estrogen receptor 1; ESR; estrogen receptor; Era; NR3A1; ER-alpha; estradiol receptor; estrogen nuclear receptor alpha; estrogen receptor alpha delta 4 +49 isoform; nuclear receptor subfamily 3 group A member 1; estrogen receptor alpha 3*,4,5,6,7*/822 isoform; estrogen receptor alpha delta 4*,5,6,7*/654 isoform; estrogen receptor alpha delta 4*,5,6,7,8*/901 isoform; estrogen receptor alpha delta 3*,4,5,6,7*/819-2 isoform; estrogen receptor alpha delta 3*,4,5,6,7*,8*/941 isoform; ER; ESRA; DKFZp686N23123
• Gene ID: 2099
• mRNA Refseq: NM_000125
• Protein Refseq: NP_000116
• UniProt ID: P03372
• Chromosome Location: 6q24-q27
• Pathway: Androgen Receptor Signaling Pathway, organism-specific biosystem; Endocrine and other factor-regulated calcium reabsorption, organism-specific biosystem; Endocrine and other factor-regulated calcium reabsorption, conserved biosystem; Estrogen signaling pathway, organism-specific biosystem; FOXA1 transcription factor network, organism-specific biosystem; FOXM1 transcription factor network, organism-specific biosystem; Gene Expression, organism-specific biosystem
• Function: DNA binding; beta-catenin binding; chromatin binding; core promoter sequence-specific DNA binding; enzyme binding; enzyme binding; estrogen receptor activity; estrogen receptor activity; estrogen response element binding; hormone binding; identical protein binding; ligand-activated sequence-specific DNA binding RNA polymerase II transcription factor activity; metal ion binding; nitric-oxide synthase regulator activity; protein binding; protein complex binding; receptor activity; sequence-specific DNA binding; sequence-specific DNA binding transcription factor activity; sequence-specific DNA binding transcription factor activity; steroid binding; steroid hormone receptor activity; steroid hormone receptor activity; transcription factor binding; type 1 metabotropic glutamate receptor binding; zinc ion binding

Reviews

05/30/2021

Its high specificity and robust activity make it an ideal candidate for investigating intricate molecular interactions and elucidating complex biological mechanisms.

04/24/2019

Tthe manufacturer's commitment to exceptional technical support offers me peace of mind, knowing that any challenges or concerns I may encounter will be effectively addressed. he manufacturer's commitment to exceptional technical support offers me peace of mind, knowing that any challenges or concerns I may encounter will be effectively addressed.

12/08/2017

With the ESR1 protein, I can confidently pursue my scientific objectives and overcome experimental obstacles, enabling me to make significant strides in my field of study.

Q&As

What are the clinical implications of ESR1 mutations in breast cancer?

ESR1 mutations can lead to resistance to endocrine therapies in breast cancer patients, impacting treatment outcomes.

How does ESR1 testing impact the prognosis of breast cancer patients?

ESR1 testing can provide important prognostic information by helping to predict the likelihood of a favorable response to hormone therapy.

How is ESR1 protein detected in clinical practice?

ESR1 protein can be detected through various laboratory techniques, including immunohistochemistry (IHC) and immunoblotting.

Can ESR1 mutations be targeted in breast cancer treatment?

Research is ongoing, and there are efforts to develop targeted therapies for ESR1-mutated breast cancer, but it's still an evolving field.

How is ESR1 status determined in breast cancer patients?

ESR1 status is determined through the examination of tumor tissue samples, often using IHC or molecular testing.