Active Recombinant Human FAP Protein, hFc-tagged

• Cat.No.: FAP-372H
• Product Overview: Recombinant Human FAP Protein(Q12884-1)(Leu26-Asp760) is expressed from Expi293 with hFc tag at the N-terminal.

Specification

• Source: HEK293
• Species: Human
• Tag: hFc
• Form: Supplied as 0.22μm filtered solution in PBS (pH 7.4). Please dilute to the desired concentration according to the concentration of the solution shown on the product label.
• Bio-activity: Immobilized Human FAP, hFc Tag at 2μg/ml (100μl/Well) on the plate. Dose response curve for Biotinylated Anti-FAP Antibody, hFc Tag with the EC50 of 0.15μg/ml determined by ELISA.
• Molecular Mass: The protein has a predicted MW of 112.3 kDa. Due to glycosylation, the protein migrates to 110-120 kDa based on Tris-Bis PAGE result.
• Protein length: Leu26-Asp760
• Endotoxin: Less than 1EU per μg by the LAL method.
• Purity: > 95% as determined by Tris-Bis PAGE
  > 95% as determined by HPLC
• Storage: Valid for 12 months from date of receipt when stored at -80°C.
  Recommend to aliquot the protein into smaller quantities for optimal storage. Please do not repeated freeze-thaw cycles.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.

Gene Information

• Gene Name: FAP fibroblast activation protein, alpha [ Homo sapiens ]
• Official Symbol: FAP
• Synonyms: FAP; fibroblast activation protein, alpha; seprase; DPPIV; integral membrane serine protease; 170 kDa melanoma membrane-bound gelatinase; FAPA; DKFZp686G13158
• Gene ID: 2191
• mRNA Refseq: NM_004460
• Protein Refseq: NP_004451
• MIM: 600403
• UniProt ID: Q12884