Active Recombinant Human FCGR2A Protein, His-tagged, Biotinylated
Cat.No. : | FCGR2A-260H |
Product Overview : | Biotinylated Human Fc gamma RIIA / CD32a (H131) (CDA-H82E6) is expressed from human HEK293 cells. It contains AA Ala 36 - Ile 218 (Accession # P12318-1). |
- Specification
- Gene Information
- Related Products
Source : | HEK293 |
Species : | Human |
Tag : | His |
Predicted N Terminal : | Ala 36 |
Form : | Lyophilized from sterile PBS, pH7.4, 10% Trehalose. |
Bio-activity : | The bioactivity is measured by its binding ability to Ipilimumab in a SPR assay. Immobilized Human FcGR2A / CD32a (H131), His Tag can bind Ipilimumab with affinity constant of around 2.3 uM. Measured by its binding ability in a functional ELISA against immobilized Yervoy Ipilimumab (0.5 ug/well). The linear range is 0.5-16 ng/mL. |
Molecular Mass : | This protein carries an Avi tag at the C-terminus, followed by a polyhistidine tag. The protein has a calculated MW of 24 kDa. The protein migrates as 30-38 kDa on a SDS-PAGE gel under reducing (R) condition due to glycosylation. |
Endotoxin : | Less than 1.0 EU per μg by the LAL method. |
Purity : | >90% as determined by SDS-PAGE. |
Stability : | Samples are stable for up to twelve months from date of receipt at -70ºC. |
Storage : | Store it under sterile conditions at -20ºC~-70ºC. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles. |
Reconstitution : | It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4℃ before opening to recover the entire contents. |
Gene Name : | FCGR2A Fc fragment of IgG, low affinity IIa, receptor (CD32) [ Homo sapiens ] |
Official Symbol : | FCGR2A |
Synonyms : | FCGR2A; Fc fragment of IgG, low affinity IIa, receptor (CD32); Fc fragment of IgG, low affinity IIa, receptor for (CD32) , FCG2, FCGR2, FCGR2A1; low affinity immunoglobulin gamma Fc region receptor II-a; CD32; CD32A; CDw32; IGFR2; Immunoglobulin G Fc receptor II; fcRII-a; fc-gamma-RIIa; fc-gamma RII-a; igG Fc receptor II-a; FCG2; FcGR; FCGR2; FCGR2A1; MGC23887; MGC30032; |
Gene ID : | 2212 |
mRNA Refseq : | NM_001136219 |
Protein Refseq : | NP_001129691 |
MIM : | 146790 |
UniProt ID : | P12318 |
Chromosome Location : | 1q23 |
Pathway : | Fc gamma R-mediated phagocytosis, organism-specific biosystem; Fc gamma R-mediated phagocytosis, conserved biosystem; Leishmaniasis, organism-specific biosystem; Leishmaniasis, conserved biosystem; Osteoclast differentiation, organism-specific biosystem; Osteoclast differentiation, conserved biosystem; Phagosome, organism-specific biosystem; |
Function : | IgG binding; receptor activity; |
Products Types
◆ Recombinant Protein | ||
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FCGR2A-3992H | Recombinant Human FCGR2A Protein, GST-tagged | +Inquiry |
FCGR2A-06H | Active Recombinant Human FCGR2A Protein, His-tagged | +Inquiry |
FCGR2A-543H | Recombinant Human FCGR2A Protein (H167) | +Inquiry |
◆ Lysates | ||
FCGR2A-678HCL | Recombinant Human FCGR2A cell lysate | +Inquiry |
FCGR2A-1926CCL | Recombinant Cynomolgus FCGR2A cell lysate | +Inquiry |
FCGR2A-1996HCL | Recombinant Human FCGR2A cell lysate | +Inquiry |
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For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.
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