| Source : |
HEK293 |
| Species : |
Human |
| Tag : |
His/Strep II |
| Predicted N Terminal : |
Ala 24(FCGRT) & Ile 21(B2M) |
| Form : |
Lyophilized from 0.22 um filtered solution in PBS, pH7.4, 10% trehalose. |
| Bio-activity : |
Immobilized Yervoy Ipilimumab (Human IgG1) at 10 ug/mL (100 uL/well) can bind Biotinylated Human FcRn / FCGRT & B2M with a linear range of 0.02-0.15 ug/mL. Immobilized Biotinylated Human FcRn / FCGRT & B2M Heterodimer Protein on SA Biosensor, can bind Herceptin with an affinity constant of 0.49 uM as determined in BLI assay (Fortebio Octet 96). |
| Molecular Mass : |
The protein has a calculated MW of 31.2 kDa (FCGRT), 13.1 kDa (B2M) . The protein migrates as 33 kDa (FCGRT), 13 kDa (B2M) respectively on a SDS-PAGE gel under reducing condition due to glycosylation. |
| Endotoxin : |
< 1.0 EU per μg of the protein as determined by the LAL method. |
| Purity : |
>95% as determined by SDS-PAGE. |
| Characteristic : |
The primary amines in the side chains of lysine residues and the N-terminus of the protein are conjugated with biotins using standard chemical labeling method. A standard biotin reagent (13.5 angstroms) is used in this product. The biotin to protein ratio is 2.5-4.5 as determined by the HABA assay. |
| Stability : |
Samples are stable for up to twelve months from date of receipt at -70ºC. |
| Storage : |
For long term storage, the product should be stored at lyophilized state at -20ºC or lower. Please avoid repeated freeze-thaw cycles. No activity loss is observed after storage at: 4-8ºC for 12 months in lyophilized state; -70ºC for 3 months under sterile conditions after reconstitution. |
| Reconstitution : |
It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4℃ before opening to recover the entire contents. |
| Official Symbol : |
FCGRT; B2M |
| Synonyms : |
FcRn, FCGRT & B2M |
