Active Recombinant Human FGF7 Protein, Pre-aliquoted

• Cat.No.: FGF7-18H
• Product Overview: Recombinant Human FGF7 Protein, Cys32 - Thr194, with an N-terminal Met, was expressed in E. coli.

Specification

• Description: The protein encoded by this gene is a member of the fibroblast growth factor (FGF) family. FGF family members possess broad mitogenic and cell survival activities, and are involved in a variety of biological processes, including embryonic development, cell growth, morphogenesis, tissue repair, tumor growth and invasion. This protein is a potent epithelial cell-specific growth factor, whose mitogenic activity is predominantly exhibited in keratinocytes but not in fibroblasts and endothelial cells. Studies of mouse and rat homologs of this gene implicated roles in morphogenesis of epithelium, reepithelialization of wounds, hair development and early lung organogenesis.
• Source: E. coli
• Species: Human
• Bio-activity: Measured in a cell proliferation assay using 4MBr‑5 rhesus monkey epithelial cells. The ED50 for this effect is 6-60 ng/mL. None
• Molecular Mass: Predicted Molecular Mass:19 kDa
  SDS-PAGE:
• Protein length: Cys32-Thr194, with an N-terminal Met
• N-terminal Sequence Analysis: Met
• Endotoxin: < 0.10 EU/μg of the protein by the LAL method.
• Purity: > 97% by SDS-PAGE under reducing conditions and visualized by silver stain.
• Stability: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 6 months from date of receipt at room temperature.
  • 12 months from date of receipt at 2-8 centigrade as supplied.
  • 1 month at 2-8 centigrade under sterile conditions after reconstitution.
  • 3 months at -20 to -80 centigrade under sterile conditions after reconstitution.
• Reconstitution: For a stock solution, reconstitute at 100 μg/mL in sterile PBS, or simply roll Pre-aliquoted directly into cell culture medium for immediate use.
• Shipping: The product is shipped at ambient temperature.

Gene Information

• Gene Name: FGF7 fibroblast growth factor 7 [ Homo sapiens (human) ]
• Official Symbol: FGF7
• Synonyms: FGF7; fibroblast growth factor 7; KGF; HBGF-7; fibroblast growth factor 7; FGF-7; heparin-binding growth factor 7; keratinocyte growth factor
• Gene ID: 2252
• mRNA Refseq: NM_002009
• Protein Refseq: NP_002000
• MIM: 148180
• UniProt ID: P21781