| Description : |
Fibroblast Growth Factor-8 (FGF-8) is a heparin-binding growth factor of the FGF family. There are 4 known forms of FGF8 produced by alternative splicing: FGF8a, FGF-8b, FGF-8e and FGF-8f. The human and mouse FGF8b are identical of aa sequences. FGF-8 plays an important role in the regulation of embryonic development, cell proliferation, cell differentiation and cell migration. FGF-8 is required for normal brain, eye, ear and limb development during embryogenesis. It is also required for normal development of the gonadotropin-releasing hormone (GnRH) neuronal system. |
| Source : |
E. coli |
| Species : |
Human |
| Form : |
Sterile Filtered White lyophilized (freeze-dried) powder. |
| Bio-activity : |
ED50 < 5.0 ng/mL, measured by a cell proliferation assay using 3T3 cells in the presence of 1 μg/mL of heparin, corresponding to a specific activity of > 2.0 × 10^5 units/mg. |
| Molecular Mass : |
22.5kDa, observed by reducing SDS-PAGE. |
| Protein Length : |
194 |
| AA Sequence : |
MQVTVQSSPNFTQHVREQSLVTDQL SRRLIRTYQLYSRTSGKHVQVLANK RINAMAEDGDPFAKLIVETDTFGSR VRVRGAETGLYICMNKKGKLIAKSN GKGKDCVFTEIVLENNYTALQNAKY EGWYMAFTRKGRPRKGSKTRQHQRE VHFMKRLPRGHHTTEQSLRFEFLNY PPFTRSLRGSQRTWAPEPR |
| Endotoxin : |
< 0.2 EU/μg, determined by LAL method. |
| Purity : |
> 95% by SDS-PAGE and HPLC analyses. |
| Storage : |
Lyophilized recombinant human Fibroblast Growth Factor-8 (rhFGF-8) remains stable up to 6 months at -80 centigrade from date of receipt. Upon reconstitution, rhFGF-8 should be stable up to 2 weeks at 4 centigrade or up to 3 months at -20 centigrade. |
| Storage Buffer : |
Lyophilized after extensive dialysis against PBS. |
| Reconstitution : |
Reconstituted in ddH2O at 100 μg/mL. |
