Active Recombinant Human FN1 Protein, His-tagged

• Cat.No.: FN1-882H
• Product Overview: Recombinant Human FN1 (Accession # NP_997647) Val1913-Glu2477, fused with a C-terminal 6-His tag, was produced in Mouse myeloma cell line, NS0-derived.

Specification

• Source: Mammalian cells
• Species: Human
• Tag: His
• Predicted N Terminal: Val1913
• Form: Lyophilized from a 0.2 µm filtered solution in PBS.
• Bio-activity: Measured by the ability of the immobilized protein to support the adhesion of B16-F1 mouse melanoma cells.When 5 x 10^4 cells/well are added to rhFN1.4 coated plates (3 µg/mL and 100 µL/well), approximately 60 - 80% will adhere after 30 minutes at 37° C.
• Molecular Mass: Recombinant Human FN1, His-tagged has a calculated MW of 63.5 kDa. In SDS-PAGE migrates as 70-95 kDa, reducing conditions.
• Protein length: Val1913-Glu2477
• Purity: >95%, by SDS-PAGE under reducing conditions and visualized by silver stain.
• Storage: Avoid repeated freeze-thaw cycles. No activity loss was observed after storage at: In lyophilized state for 1 year (4ºC); After reconstitution under sterile conditions for 3 months (-70ºC).

Gene Information

• Gene Name: FN1 fibronectin 1 [ Homo sapiens ]
• Official Symbol: FN1
• Synonyms: FN1; fibronectin 1; fibronectin; CIG; cold insoluble globulin; FINC; GFND2; LETS; migration stimulating factor; MSF; cold-insoluble globulin; migration-stimulating factor; FN; FNZ; ED-B; GFND; DKFZp686H0342; DKFZp686I1370; DKFZp686F10164; DKFZp686O13149
• Gene ID: 2335
• mRNA Refseq: NM_002026
• Protein Refseq: NP_002017
• MIM: 135600
• UniProt ID: P02751
• Chromosome Location: 2q34