Active Recombinant Human Frizzled Family Receptor 8, Fc-tagged
Cat.No. : | FZD8-597H |
Product Overview : | RecombinantHuman FZD8 protein was expressed in Chinese Hamster Ovary cell line with theFc part of Human IgG1 Pro100-Lys330 (C-terminus) and Human Frizzled-8 Ala25-Pro172(N-terminus). |
- Specification
- Gene Information
- Related Products
Cat. No. : | FZD8-597H |
Description : | FZD8 is one of atleast ten seventransmembrane (7TM) glycoprotein of the Frizzled family of Wntreceptors. Frizzled proteins are thought to be G-protein-coupled. Wnt engagement,with low density lipoprotein receptor related proteins LRP-5 or LRP-6 actingas co-receptors stabilizes β-catenin and promotes gene transcription that isimportant in development and tissue maintenance. |
Source : | Chinese HamsterOvary cell line, CHO-derived |
Form : | Supplied as a 0.2 μmfiltered solution in PBS. |
N-terminal Sequence : | Ala25 & Ala28 |
Molecular Weight : | 43.3 kDa |
Activity : | Measured by itsbinding ability in a functional ELISA. In a 100 μL reaction mixturecontaining biotinylated rmWnt-3a or rmWnt-5a at 50-100 ng/mL and rhFrizzled8/Fc Chimera dilutions at 0.1-8,000 ng/mL, the concentration of rhFrizzled8/ FcChimera that produces 50% of the optimal binding response is found to beapproximately 7-28 ng/mL. |
Endotoxin Level : | <1.0 EU per 1 μgof the protein by the LAL method. |
SDS-PAGE : | 55-65 kDa, reducingconditions |
Purity : | >95%, by SDS-PAGEunder reducing conditions and visualized by silver stain. |
Reconstitution : | Reconstitute at 500μg/mL in sterile PBS. |
Storage : | Use amanual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1month, 2 to 8 °C under sterile conditions after reconstitution.3 months, -20 to-70 °C under sterile conditions after reconstitution. |
Gene Name : | FZD8 frizzled family receptor 8[ Homo sapiens ] |
Official Symbol : | FZD8 |
Synonyms : | FZD8; frizzledfamily receptor 8; FZ-8; hFZ8; frizzled (Drosophila) homolog 8;frizzled 8, seven transmembrane spanning receptor; frizzled homolog 8(Drosophila); OTTHUMP00000019454; hFz8; Fz-8; frizzled-8; frizzledhomolog 8 |
Gene ID : | 8325 |
mRNA Refseq : | NM_031866 |
Protein Refseq : | NP_114072 |
MIM : | 606146 |
UniProt ID : | Q9H461 |
Chromosome Location : | 10p11.2 |
Pathway : | Basal cellcarcinoma; Class B/2 (Secretin family receptors); GPCR ligand binding; HTLV-Iinfection; Melanogenesis; Pathways in cancer; Signal Transduction; Signalingby GPCR; Wnt Signaling Pathway; Wnt signaling network |
Function : | G-proteincoupled receptor activity; PDZ domain binding; Wnt-activated receptoractivity; Wnt-protein binding |
Products Types
◆ Recombinant Protein | ||
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FZD8-596H | Recombinant Human FZD8(Ala28-Pro172) Protein, C-6*His-tagged | +Inquiry |
Related Gene
For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.
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