Active Recombinant Human GBA, MYC/DDK-tagged
Cat.No. : | GBA-020H |
Product Overview : | Recombinant Human GBA, fused with C-terminal MYC/DDK, was expressed in HEK293 cells. |
- Specification
- Gene Information
- Related Products
Description : | This gene encodes a lysosomal membrane protein that cleaves the beta-glucosidic linkage of glycosylceramide, an intermediate in glycolipid metabolism. Mutations in this gene cause Gaucher disease, a lysosomal storage disease characterized by an accumulation of glucocerebrosides. A related pseudogene is approximately 12 kb downstream of this gene on chromosome 1. Alternative splicing results in multiple transcript variants. |
Source : | HEK293 cells |
Species : | Human |
Tag : | MYC/DDK |
Bio-activity : | The enzymatic activity of GBA was measured by its ability to hydrolyze a fluorescent substrate 4-methylumbelliferyl-ß-D-glucopyranoside. The specific activity is > 70,000 pmol/hour/µg, as measured under the following conditions: 27 ng of GBA was incubated with 10 mM 4-methylumbelliferyl- ß-D-glucopyranoside in the following buffer at 37°C for 40 min: 150 mM citrate-phosphate buffer, pH 5.4, 0.25% (w/w) sodium taurocholate, 0.25% (w/w) Triton X-100, and 1% bovine serum albumin. The reaction was terminated by adding 0.5 volume of 1M glycine buffer, pH 12.5. The hydrolyzed product of reaction, 4-methylumbelliferone (4-MU), was measured using a FlexStation 3 microplate reader (Ex365/Em445). Specific activity of GBA was calculated based on a standard curve of known concentration of 4-MU. |
Molecular Mass : | 55.5 kDa |
Purity : | > 80% as determined by SDS-PAGE and Coomassie blue staining |
Concentration : | >50 ug/mL as determined by microplate BCA method |
Storage Buffer : | 25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10% glycerol. |
Gene Name : | GBA glucosidase, beta, acid [ Homo sapiens ] |
Official Symbol : | GBA |
Synonyms : | GBA; GCB; GBA1; GLUC; glucosidase, beta, acid; glucosylceramidase; beta-GC; alglucerase; imiglucerase; acid beta-glucosidase; beta-glucocerebrosidase; lysosomal glucocerebrosidase; D-glucosyl-N-acylsphingosine glucohydrolase; NP_000148.2; EC 3.2.1.45; NP_001005741.1; NP_001005742.1; NP_001165282.1; NP_001165283.1 |
Gene ID : | 2629 |
mRNA Refseq : | NM_000157 |
Protein Refseq : | NP_000148 |
MIM : | 606463 |
UniProt ID : | P04062 |
Chromosome Location : | 1q21 |
Pathway : | Glycosphingolipid metabolism; Other glycan degradation; Sphingolipid metabolism |
Function : | glucosylceramidase activity; protein binding; receptor binding |
Products Types
◆ Recombinant Protein | ||
GBA-3492M | Recombinant Mouse GBA Protein, His (Fc)-Avi-tagged | +Inquiry |
GBA-4764H | Recombinant Human GBA Protein, GST-tagged | +Inquiry |
GBA-539H | Recombinant Human GBA Protein (Ala40-Gln536), His-tagged | +Inquiry |
Gba-3165M | Recombinant Mouse Gba Protein, Myc/DDK-tagged | +Inquiry |
Gba-7842M | Recombinant Mouse Gba protein, His & GST-tagged | +Inquiry |
◆ Lysates | ||
GBA-6002HCL | Recombinant Human GBA 293 Cell Lysate | +Inquiry |
Related Gene
For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.
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