Active Recombinant Human GCK(1-473) Protein, N-GST-tagged

• Cat.No.: GCK-93H
• Product Overview: N-terminal GSTtagged, recombinant, human GCK amino acids 1–473, expressed by baculovirus in Sf21 insect cells. Purified using glutathione agarose

Specification

• Description: GCK (Glucokinase) is a member of the hexokinase family of proteins. Hexokinases phosphorylate glucose to produce glucose-6-phosphate, the first step in most glucose metabolism pathways. In contrast to other forms of hexokinase, this enzyme is not inhibited by its product glucose-6-phosphate but remains active while glucose is abundant. The use of multiple promoters and alternative splicing of this gene result in distinct protein isoforms that exhibit tissue-specific expression in the pancreas and liver. In the pancreas, this enzyme plays a role in glucose-stimulated insulin secretion, while in the liver, this enzyme is important in glucose uptake and conversion to glycogen. Mutations in this gene that alter enzyme activity have been associated with multiple types of diabetes and hyperinsulinemic hypoglycemia.
• Source: Sf21
• Species: Human
• Tag: GST
• Protein length: 1-473 aa
• Form: 0.759mg/ml of enzyme in 50mM Tris/HCl pH7.5, 300mM NaCl, 0.1mM EGTA, 0.03% Brij-35, 270mM sucrose, 1mM benzamidine, 0.2mM PMSF, 0.1% 2-mercaptoethanol. Frozen solution.
• Bio-activity: 2100U/mg, where one unit of GCK, active activity is defined as 1nmol phosphate incorporated into 0.8mg/ml myelin basic protein per minute at 30 centigrade with a final ATP concentration of 100μM.
• Molecular Mass: 80.6 kDa
• AA Sequence: 1 MSPILGYWKI KGLVQPTRLL LEYLEEKYEE HLYERDEGDK WRNKKFELGL EFPNLPYYID 61 GDVKLTQSMA IIRYIADKHN MLGGCPKERA EISMLEGAVL DIRYGVSRIA YSKDFETLKV 121 DFLSKLPEML KMFEDRLCHK TYLNGDHVTH PDFMLYDALD VVLYMDPMCL DAFPKLVCFK 181 KRIEAIPQID KYLKSSKYIA WPLQGWQATF GGGDHPPKSD LEVLFQGPEF KGLRRQMALL 241 RDVSLQDPRD RFELLQRVGA GTYGDVYKAR DTVTSELAAV KIVKLDPGDD ISSLQQEITI 301 LRECRHPNVV AYIGSYLRND RLWICMEFCG GGSLQEIYHA TGPLEERQIA YVCREALKGL 361 HHLHSQGKIH RDIKGANLLL TLQGDVKLAD FGVSGELTAS VAKRRSFIGT PYWMAPEVAA 421 VERKGGYNEL CDVWALGITA IELGELQPPL FHLHPMRALM LMSKSSFQPP KLRDKTRWTQ 481 NFHHFLKLAL TKNPKKRPTA EKLLQHPFTT QQLPRALLTQ LLDKASDPHL GTPSPEDCEL 541 ETYDMFPDTI HSRGQHGPAE RTPSEIQFHQ VKFGAPRRKE TDPLNEPWEE EWTLLGKEEL 601 SGSLLQSVQE ALEERSLTIR SASEFQELDS PDDTMGTIKR APFLGPLPTD PPAEEPLSSP 661 PGPNSSPLLP TAWATMKQRE DPERSSCHGL PPTPKVHMGA CFSKVFNGC
• Purity: Purity 54.2% by SDS-PAGE and Coomassie blue staining.
• Applications: Drug Discovery & Development
• Storage: 6 months at -70 centigrade.
• Handling Advice: Rapidly thaw the vial under cold water and immediately place on ice. Aliquot unused material into prechilled micro-centrifuge tubes and immediately snap-freeze the vials in liquid nitrogen prior to re-storage at -70 centigrade.
• Use/Stability: On receipt of material store at -70 centigrade. Unopened reagent is stable for a minimum of 1 year from date of shipment when stored at recommended storage temperature. Avoid repeat freeze/thaw cycles. For maximum recovery of product, centrifuge original vial prior to removing the cap.

Gene Information

• Gene Name: GCK glucokinase [ Homo sapiens (human) ]
• Official Symbol: GCK
• Synonyms: GCK; glucokinase; GK; GLK; HK4; HHF3; HKIV; HXKP; LGLK; MODY2; PNDM1; FGQTL3
• Gene ID: 2645
• mRNA Refseq: NM_000162.5
• Protein Refseq: NP_000153.1
• MIM: 138079
• UniProt ID: P35557