Active Recombinant Human GDF5 Protein (Ala382-Arg501), C-His tagged, Animal-free, Carrier-free

• Cat.No.: GDF5-299H
• Product Overview: Recombinant Human GDF5 Protein (Ala382-Arg501) with C-His tag was expressed in E. coli and Animal-free as well as Carrier-free.

Specification

• Description: Bone Morphogenetic Protein-14 (BMP-14), known as Growth differentiation factor 5 (GDF5), is an extracellular multifunctional cytokine that is also a member of the TGFβ family. BMP-14 can bind with the TGFβ receptor and trigger SMAD protein signal transduction. BMP-14 plays a role in skeletal and joint development and increases the survival of neurons that respond to the neurotransmitter dopamine.
• Source: E. coli
• Species: Human
• Tag: C-His
• Form: Lyophilized
• Bio-activity: Measure by its ability to induce alkaline phosphatase production by ATDC5 cells. The ED50 for this effect is < 14 ng/mL.
• Molecular Mass: The protein has a calculated MW of 14.52 kDa.
  The protein migrates as 18 kDa under reducing condition (SDS-PAGE analysis).
• Protein Length: Ala382-Arg501
• Endotoxin: < 0.1 EU/μg of the protein by the LAL method.
• Purity: >98% as determined by SDS-PAGE analysis.
• Storage: This product is stable after storage at:
  -20 centigrade for 12 months in lyophilized state from date of receipt.
  -20 or -80 centigrade for 1 month under sterile conditions after reconstitution.
  Avoid repeated freeze/thaw cycles.
• Storage Buffer: Lyophilized from a 0.2 μm filtered solution containing 20 mM sodium citrate and 0.2 M NaCl, pH 3.5.
• Reconstitution: It is recommended to reconstitute the lyophilized protein in 4 mM HCl to a concentration not less than 200 μg/mL and incubate the stock solution for at least 20 min to ensure sufficient re-dissolved.
• Shipping: The product is shipped with polar packs. Upon receipt, store it immediately at -20 centigrade or lower for long term storage.

Gene Information

• Gene Name: GDF5 growth differentiation factor 5 [ Homo sapiens (human) ]
• Official Symbol: GDF5
• Synonyms: GDF5; growth differentiation factor 5; growth/differentiation factor 5; BMP14; cartilage derived morphogenetic protein 1; CDMP1; GDF-5; CDMP-1; radotermin; cartilage-derived morphogenetic protein-1; OS5; LAP4; SYNS2
• Gene ID: 8200
• mRNA Refseq: NM_000557
• Protein Refseq: NP_000548
• MIM: 601146
• UniProt ID: P43026