| Source : |
HEK293 |
| Species : |
Human |
| Tag : |
Flag-His |
| Form : |
Supplied as 0.2 μm filtered solution in 50 mM HEPES, 150 mM NaCl, pH7.5 with glycerol as protectant. |
| Bio-activity : |
Immobilized Human GPRC5D Protein, Flag,His Tag at 10 μg/mL (100 μL/well) can bind Anti-GPRC5D Antibody, Human IgG4 with a linear range of 0.01-0.08 μg/mL. |
| Molecular Mass : |
This protein carries a Flag tag at the N-terminus and a polyhistidine tag at the C-terminus with calculated MW of 51.8 kDa and migrates as 35 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation. The membrane scaffold protein (MSP1D1) has calculated MW of 24.7 kDa, and it migrates as 25 kDa under reducing (R) condition (SDS-PAGE).Nanodiscs are a new class of model membranes that are being used to solubilize and study a range of integral membrane proteins and membrane-associated proteins. The Nanodisc bilayer is bounded by a membrane scaffold protein (MSP1D1) coat that confers enhanced stability and a narrow particle size distribution. The nanodisc assembles from a mixture of full length membrane protein in detergent, phospholipid micelles and membrane scaffold protein(MSP1D1) upon removal of the detergent. |
| Proteinlength : |
Tyr2-Phe192&Glu197-Leu262 |
| Endotoxin : |
Less than 1.0 EU per μg by the LAL method. |
| Purity : |
>90% as determined by SDS-PAGE. |
| Storage : |
For long term, the product should be stored at lyophilized state at -20°C or lower.Please avoid repeated freeze-thaw cycles. This product is stable after at:
-20°C to -70°C for 12 months in lyophilized state; -70°C for 3 months under sterile conditions after reconstitution. |
| Reconstitution : |
It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents. |
