Active Recombinant Human GZMA protein, His-tagged

• Cat.No.: GZMA-01H
• Product Overview: Recombinant human Granzyme A protein Cys26-Val262 was expressed in NS0, with a C-terminal 10-His tag.

Specification

• Description: Cytolytic T lymphocytes (CTL) and natural killer (NK) cells share the remarkable ability to recognize, bind, and lyse specific target cells. They are thought to protect their host by lysing cells bearing on their surface 'nonself' antigens, usually peptides or proteins resulting from infection by intracellular pathogens. The protein described here is a T cell- and natural killer cell-specific serine protease that may function as a common component necessary for lysis of target cells by cytotoxic T lymphocytes and natural killer cells.
• Source: NS0
• Species: Human
• Tag: His
• Form: Lyophilized from a 0.2 μm filtered solution in MES, NaCl and CaCl2 with Trehalose.
• Bio-activity: Measured by its ability to cleave a colorimetric peptide substrate, N-carbobenzyloxy-Gly-Arg-ThioBenzyl ester (Z-GR-SBzl), in the presence of 5,5’Dithio-bis (2-nitrobenzoic acid) (DTNB). The specific activity is >5,000 pmol/min/µg, as measured under the described conditions.
• Molecular Mass: Predicted Molecular Mass: 28 kDa
  SDS-PAGE: Multiple bands between 29-33 kDa, reducing conditions
• N-terminal Sequence Analysis: Cys26
• Endotoxin: <1.0 EU per 1 μg of the protein by the LAL method.
• Purity: >95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  6 months from date of receipt, -20 to -70 centigrade as supplied.
  3 months, -20 to -70 centigrade under sterile conditions after reconstitution.
• Reconstitution: Reconstitute at 100 μg/mL in sterile 25 mM HEPES, 150 mM NaCl and 10 mM CaCl2, pH 7.5.
• Shipping: The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Gene Information

• Gene Name: GZMA granzyme A [ Homo sapiens (human) ]
• Official Symbol: GZMA
• Synonyms: CTL Tryptase; CTLA3; CTLA3Granzyme A (Cytotoxic T-lymphocyte-associated serine esterase-3; Hanukah factorserine protease); Cytotoxic T-lymphocyte proteinase 1; EC 3.4.21; Fragmentin-1; granzyme A (granzyme 1, cytotoxic T-lymphocyte-associated serine esterase 3); Granzyme A; Granzyme-1; GZMA; H factor; Hanukkah factor; HF; HFSP; HFSPEC 3.4.21.78
• Gene ID: 3001
• mRNA Refseq: NM_006144
• Protein Refseq: NP_006135
• MIM: 140050
• UniProt ID: P12544