Active Recombinant Human HSPA8, His-tagged

• Cat.No.: HSPA8-28200TH
• Product Overview: Recombinant Human HSPA8, fussed with His tag, was expressed in E. coli

Specification

• Description: Heat shock 70 kDa protein 8 also known as heat shock cognate 71 kDa protein or Hsc70 or Hsp73 is a heat shock protein that in humans is encoded by the HSPA8 gene. As a member of the heat shock protein 70 family and a chaperone protein, it facilitates the proper folding of newly translated and misfolded proteins, as well as stabilize or degrade mutant proteins. Its functions contribute to biological processes including signal transduction, apoptosis, protein homeostasis, and cell growth and differentiation. It has been associated with an extensive number of cancers, neurodegenerative diseases, cell senescence and aging.
• Source: E. coli
• Species: Human
• Tag: His
• Form: 20mM Tris/HCl pH7.5, 1mM EDTA.
• Bio-activity: The protein has ATPase activity at the time of manufacture of 3.2μM phosphate liberated/hr/μg protein in a 200μl reaction at 37°C (pH 8) in the presence of 20ul of 1mM ATP using a Malachite Green assay.
• Molecular Mass: Molecular weight of approximately 73kDa
• Purity: >90% pure using SDS-PAGE analysis.
• Applications: ATPase Assay, WB control, Binding Assay, ELISA reference standard
• Stability: Store at -20°C; more than 1 year. Avoid freeze/ thaw cycle.
• Concentration: 2.1 mg/mL

Gene Information

• Gene Name: HSPA8 heat shock 70kDa protein 8 [ Homo sapiens ]
• Official Symbol: HSPA8
• Synonyms: LAP1; HSC54; HSC70; HSC71; HSP71; HSP73; LAP-1; NIP71; HEL-33; HSPA10; HEL-S-72p; heat shock cognate 71 kDa protein; LPS-associated protein 1; N-myristoyltransferase inhibitor protein 71; constitutive heat shock protein 70; epididymis luminal protein 33; epididymis secretory sperm binding protein Li 72p; heat shock 70kd protein 10; heat shock cognate protein 54; lipopolysaccharide-associated protein 1
• Gene ID: 3312
• mRNA Refseq: NM_006597
• Protein Refseq: NP_006588
• MIM: 600816
• UniProt ID: P11142
• Chromosome Location: 11q24.1
• Pathway: AUF1 (hnRNP D0) destabilizes mRNA, organism-specific biosystem; Antigen processing and presentation, conserved biosystem; Developmental Biology, organism-specific biosystem
• Function: ATP binding; ATPase activity; C3HC4-type RING finger domain binding

Reviews

12/01/2021

The lab product's precision and quality are noteworthy.

10/25/2020

The customer service team was accommodating, and the delivery exceeded my expectations.

05/10/2018

This lab product is synonymous with excellence.

Q&As

In the context of cellular stress, how does HSPA8 contribute to the heat shock response, and what downstream signaling pathways does it modulate to promote cell survival?

HSPA8 is a central player in the heat shock response, assisting in the refolding of denatured proteins. It modulates downstream pathways such as HSF1 activation, orchestrating a cellular survival response.

Can you discuss the involvement of HSPA8 in neurodegenerative diseases, and how does its chaperone activity influence protein aggregation and neuronal dysfunction?

HSPA8 plays a role in neurodegenerative diseases, mitigating protein aggregation and neuronal dysfunction. Its chaperone activity is crucial for maintaining protein homeostasis in the nervous system.

How does HSPA8 participate in the regulation of apoptosis, and what anti-apoptotic mechanisms does it employ to maintain cellular viability under stress conditions?

HSPA8 exhibits anti-apoptotic functions by interacting with pro-apoptotic proteins and preventing their activation. Its role in apoptosis regulation contributes to cell survival during stress.

What is the significance of HSPA8 in autophagy, and how does it influence the selective degradation of misfolded proteins and damaged organelles?

HSPA8 is involved in autophagy, facilitating the clearance of misfolded proteins and damaged organelles. It interacts with autophagy receptors, promoting their recognition by autophagosomes.

What are the mechanisms by which HSPA8 regulates the immune response, particularly its involvement in antigen presentation and the modulation of immune cell functions?

HSPA8 participates in antigen presentation and modulates immune cell functions. It chaperones antigens for presentation on major histocompatibility complexes, influencing immune recognition and response.