Active Recombinant Human IDH1 protein, His-tagged, SEC-HPLC verified

• Cat.No.: IDH1-2527H
• Product Overview: Active Recombinant Human IDH1 protein(Met 1 - Leu 414)(O75874-1), fused with C-terminal His tag, was expressed in E. coli.

Specification

• Source: E. coli
• Species: Human
• Tag: C-His
• Protein length: Met 1 - Leu 414
• Form: Supplied as 0.2 μm filtered solution in 50 mM Tris, 150 mM NaCl, 1 mM TCEP, pH7.5 with glycerol as protectant.
• Bio-activity: Measured by the ability to oxidatively decarboxylate isocitrate to 2-oxoglutarate. The specific activity is >10000 pmol/min/μg, as measured under the described conditions.
• Molecular Mass: The protein has a calculated MW of 48.5 kDa. The protein migrates as 45-48 kDa under reducing (R) condition (SDS-PAGE).
• Endotoxin: Less than 1.0 EU per μg by the LAL method.
• Purity: >95% as determined by SDS-PAGE.
• Storage: For long term storage, the product should be stored at lyophilized state at -20°C or lower.
  Please avoid repeated freeze-thaw cycles.
  This product is stable after storage at:
  -20°C to -70°C for 12 months in lyophilized state;
  -70°C for 3 months under sterile conditions after reconstitution.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.

Gene Information

• Gene Name: idh1 isocitrate dehydrogenase (NAD+) subunit 1 Idh1 [ Schizosaccharomyces pombe 972h- ]
• Official Symbol: IDH1
• Synonyms: IDH1; isocitrate dehydrogenase (NAD+) subunit 1 Idh1; EC 1.1.1.41
• Gene ID: 2541894
• mRNA Refseq: NM_001019820
• Protein Refseq: NP_594397