Active Recombinant Human IFN-alpha 2b Protein

• Cat.No.: IFNA2-114H
• Product Overview: Recombinant Human IFN-alpha 2b Protein without tag was expressed in E. coli.

Specification

• Description: Interferon-alpha 2b (IFN-α 2b) is a type I interferon made by leukocytes during viral infection. The JAK-STAT pathway mediates the antiviral and anti-cell proliferation activities of IFN-α 2b. IFN-α proteins are widely used as standard treatments during antiviral and antineoplastic therapies. The IFN-α 2b variant differs from IFN-α 2a by one amino acid.
• Source: E. coli
• Species: Human
• Bio-activity: Viral CPE assay using EMC virus on A549 cells, ≤NA; ≥2.0 x 10^8 units/mg
• Molecular Mass: Monomer, 19.4 kDa (166 aa)
• AA Sequence: MCDLPQTHSLGSRRTLMLLAQMRRI SLFSCLKDRHDFGFPQEEFGNQFQK AETIPVLHEMIQQIFNLFSTKDSSA AWDETLLDKFYTELYQQLNDLEACV IQGVGVTETPLMKEDSILAVRKYFQ RITLYLKEKKYSPCAWEVVRAEIMR SFSLSTNLQESLRSKE
• Endotoxin: ≤1 EUs/μg, Kinetic LAL
• Purity: ≥95%, Reducing and Non-Reducing SDS PAGE
• Stability: 12 months from date of receipt when stored at -20 to -80 centigrade as supplied.
  1 month when stored at 4 centigrade after reconstituting as directed.
  3 months when stored at -20 to -80 centigrade after reconstituting as directed.
• Storage: Storage Prior to Reconstitution: -20 centigrade
• Storage Buffer: Lyophilized from a sterile (0.2 micron) filtered aqueous solution containing 0.1% Trifluoroacetic Acid (TFA)
• Reconstitution: Sterile water at 0.1 mg/mL
• Shipping: Room temperature
• Instructions: Centrifuge vial before opening. Suspend the product by gently pipetting the above recommended solution down the sides of the vial. DO NOT VORTEX. Allow several minutes for complete reconstitution. For prolonged storage, dilute to working aliquots in a 0.1% BSA solution, store at -80 centigrade and avoid repeat freeze thaws.

Gene Information

• Gene Name: IFNA2 interferon, alpha 2 [ Homo sapiens (human) ]
• Official Symbol: IFNA2
• Synonyms: IFNA2; interferon, alpha 2; interferon alpha-2; alpha 2a interferon; IFN alphaA; IFNA; interferon alpha 2b; interferon alpha A; leIF A; IFN-alpha-2; alpha-2a interferon; INFA2; IFNA2B; IFN-alphaA; MGC125764; MGC125765
• Gene ID: 3440
• mRNA Refseq: NM_000605
• Protein Refseq: NP_000596
• MIM: 147562
• UniProt ID: P01563