Active Recombinant Human IFNA8 protein(Cys24-Glu189)

• Cat.No.: IFNA8-3219H
• Product Overview: Recombinant Human IFNA8 (NP_002161.2)(Cys24-Glu189) was expressed and purified in HEK293 with two additional amino acids (Gly & Pro) at the N-terminus.

Specification

• Source: HEK293
• Species: Human
• Tag: N/A
• Protein length: Cys24-Glu189
• Form: Lyophilized from sterile PBS, pH 7.4. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
• Bio-activity: Measured in antiviral assays using WISH cells infected with vesicular stomatitisvirus (VSV). The ED50 for this effect is 0.2-2 pg/mL.
• Molecular Mass: The secreted recombinant human IFNA8 consists of 168 amino acids and predicts a molecular mass of 19.6 KDa. The apparent molecular mass of the protein is approximately 26 KDa in SDS-PAGE under reducing conditions due to glycosylation.
• Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method
• Purity: > 95 % as determined by SDS-PAGE
• Storage: Samples are stable for up to twelve months from date of receipt at -20°C to -80°C. Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.

Gene Information

• Gene Name: IFNA8 interferon, alpha 8 [ Homo sapiens ]
• Official Symbol: IFNA8
• Synonyms: IFNA8; interferon, alpha 8; interferon alpha-8; IFN alphaB; interferon alpha type 201; interferon alpha B; leIF B; IFN-alpha-8; interferon alpha-B; interferon alpha-B2; IFN-alphaB
• Gene ID: 3445
• mRNA Refseq: NM_002170
• Protein Refseq: NP_002161
• MIM: 147568
• UniProt ID: P32881