| Description : |
Interferon-gamma (IFN-γ, also known as Type II interferon or immune interferon) is a cytokine produced primarily by T-lymphocytes and natural killer cells. The protein shares no significant homology with IFN-β or the various IFN-α family proteins. Mature IFN-γ exists as noncovalently-linked homodimers. Human IFN-γ is highly species specific and is biologically active only in human and primate cells. IFN-γ was originally characterized based on its antiviral activities. The protein also exerts antiproliferative, immunoregulatory and proinflammatory activities and is thus important in host defense mechanisms. IFN-γ induces the production of cytokines, upregulates the expression of class I and II MHC antigens, Fc receptor and leukocyte adhesion molecules. It modulates macrophage effector functions, influences isotype switching and potentiates the secretion of immunoglobulins by B cells. IFN-γ also augments TH1 cell expansion and may be required for TH1 cell differentiation. IFN-γ exerts its biological activities by binding to specific cell surface receptors with high-affinity binding sites. The IFN-γ receptor is present on almost all cell types except mature erythrocytes and has been cloned and characterized. The IFN-γ receptor is structurally related to the recently cloned IL-10 receptor. |
| Source : |
E. coli |
| Species : |
Human |
| Form : |
Lyophilized from a 0.2μm filtered solution in 20mM Phosphate buffer |
| Bio-activity : |
Bioactivity is detected using WISH cell (a heteroploid human amnion cell line). The specific activity shall be not less than 2.0×10^7 IU/mg of protein. |
| Endotoxin : |
≤1EU/μg, determined by the LAL method. |
| Purity : |
≥97%, as determined by SDS-PAGE and HPLC method. |
| Usage : |
FOR RESEARCH ONLY |
| Storage : |
Lyophilized samples are stable for greater than six months from date of receipt at -20 centigrade to -70 centigrade. The reconstituted samples can be stored under sterile conditions at 2-8 centigrade for one month or at -20 centigrade to -70 centigrade for three months without detectable loss of activity. Avoid repeated freeze-thaw cycles. |
| Reconstitution : |
It is recommended that sterile ddH2O containing at least 0.1% human serum albumin or bovine serum albumin be added to the vial to prepare a stock solution of not less than 10μg/ml of the cytokine. |
