Active Recombinant Human IFNG Protein (Gln24-Gln166), C-His tagged, Animal-free, Carrier-free

• Cat.No.: IFNG-156H
• Product Overview: Recombinant Human IFNG Protein (Gln24-Gln166) with C-His tag was expressed in E. coli and Animal-free as well as Carrier-free.

Specification

• Description: The cytokine IFN gamma could protect cells from viral infections and belongs to the family of interferons. A lot of studies have shown that IFN gamma secreted by antigen triggered cell types, including T cells, naive CD4+ T cells, macrophages, dendritic cells, and B cells. IFN gamma plays an important role to trigger the macrophage act against a diverse group of microbial targets, and the pleiotropic molecule associated with antiproliferative, pro-apoptotic and antitumor mechanisms. Based on the effector cytokine considered as a major effector of immunity, it has been used in the treatment of several diseases.
• Source: E. coli
• Species: Human
• Tag: C-His
• Form: Lyophilized
• Bio-activity: Measure by its ability to induce cytotoxicity in HT29 cells. The ED50 for this effect is < 1 ng/mL. The specific activity of recombinant human IFN gamma is approximately >2 × 10⁶ IU/mg.
• Molecular Mass: The protein has a calculated MW of 17.7 kDa.
  The protein migrates as 17 kDa under reducing condition (SDS-PAGE analysis).
• Protein Length: Gln24-Gln166
• Endotoxin: < 0.01 EU/μg of the protein by the LAL method.
• Purity: >95% as determined by SDS-PAGE analysis.
• Storage: This product is stable after storage at:
  -20 centigrade for 12 months in lyophilized state from date of receipt.
  -20 or -80 centigrade for 1 month under sterile conditions after reconstitution.
  Avoid repeated freeze/thaw cycles.
• Storage Buffer: Lyophilized from a 0.2 μm filtered solution of PBS, pH 8.0.
• Reconstitution: It is recommended to reconstitute the lyophilized protein in sterile H2O to a concentration not less than 200 μg/mL and incubate the stock solution for at least 20 min to ensure sufficient re-dissolved.
• Shipping: The product is shipped with polar packs. Upon receipt, store it immediately at -20 centigrade or lower for long term storage.

Gene Information

• Gene Name: IFNG interferon, gamma [ Homo sapiens (human) ]
• Official Symbol: IFNG
• Synonyms: IFNG; interferon, gamma; interferon gamma; IFN-gamma; immune interferon; IFG; IFI
• Gene ID: 3458
• mRNA Refseq: NM_000619
• Protein Refseq: NP_000610
• MIM: 147570
• UniProt ID: P01579