Active Recombinant Human IKBKE, GST-tagged

• Cat.No.: IKBKE-125H
• Product Overview: Full length recombinant human IKK epsilon was expressed by baculovirus in Sf9 insect cells using an N-terminal GST tag.

Specification

• Description: IKBKE is a noncanonical I-kappa-B (see MIM 164008) kinase (IKK) that is essential for regulating antiviral signaling pathways. IKBKE has also been identified as a breast cancer (MIM 114480) oncogene and is amplified and overexpressed in over 30% of breast carcinomas and breast cancer cell lines.
• Source: Sf9 insect cells using baculovirus
• Species: Human
• Tag: GST
• Form: 50mM Tris-HCl, pH 7.5, 150mM NaCl, 10mM glutathione, 0.1mM EDTA, 0.25mM DTT, 0.1mM PMSF, 25% glycerol.
• Bio-activity: 12 nmol/min/mg
• Molecular Mass: 110 kDa
• Purity: >70%
• Applications: Kinase Assay; Western Blot
• Stability: 1 yr at -70°C from date of shipment.
• Storage: Store product at –70°C. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.
• Concentration: 0.05 ug/ul

Gene Information

• Gene Name: IKBKE inhibitor of kappa light polypeptide gene enhancer in B-cells, kinase epsilon [ Homo sapiens (human) ]
• Official Symbol: IKBKE
• Synonyms: IKBKE; inhibitor of kappa light polypeptide gene enhancer in B-cells, kinase epsilon; inhibitor of nuclear factor kappa-B kinase subunit epsilon; IKK i; IKKE; KIAA0151; IKK-epsilon; I-kappa-B kinase epsilon; inducible IkappaB kinase; IKK-related kinase epsilon; inducible I kappa-B kinase; IKKI; IKK-E; IKK-i; MGC125294; MGC125295; MGC125297
• Gene ID: 9641
• mRNA Refseq: NM_014002
• Protein Refseq: NP_054721
• MIM: 605048
• UniProt ID: Q14164
• Chromosome Location: 1q32.1
• Pathway: Activated TLR4 signalling; Cytosolic DNA-sensing pathway; Herpes simplex infection
• Function: ATP binding; IkappaB kinase activity; NF-kappaB-inducing kinase activity; protein binding