Active Recombinant Human ISG15 Protein (Met1-Gly156, C78S, G157C), C-6×His tagged

• Cat.No.: ISG15-11H
• Product Overview: Recombinant Human ISG15 AMC Protein (Met1-Gly156, C78S, G157C) with a C-terminal 6-His tag (C-terminal AMC derivatized protein) was expressed in E. coli.

Specification

• Description: The protein encoded by this gene is a ubiquitin-like protein that is conjugated to intracellular target proteins upon activation by interferon-alpha and interferon-beta. Several functions have been ascribed to the encoded protein, including chemotactic activity towards neutrophils, direction of ligated target proteins to intermediate filaments, cell-to-cell signaling, and antiviral activity during viral infections. While conjugates of this protein have been found to be noncovalently attached to intermediate filaments, this protein is sometimes secreted.
• Source: E. coli
• Species: Human
• Tag: C-6×His
• Bio-activity: This fluorogenic substrate for ISG15 hydrolases is based on the carboxy-terminus derivatization of ISG15 with 7-amido-4-methylcoumarin (AMC). Release of AMC fluorescence can be monitored with an excitation wavelength of 380 nm and an emission wavelength of 460 nm. Reaction conditions will need to be optimized for each specific application. We recommend an initial Recombinant Human ISG15 AMC concentration of 0.1-1 μM.
• Molecular Mass: 18 kDa
• Protein Length: Met1-Gly156
• Storage: Protect from light. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  6 months from date of receipt, -70 centigrade as supplied.
  3 months, -70 centigrade under sterile conditions after opening.
• Storage Buffer: Supplied as a 0.2 μm filtered solution in HEPES, NaCl, Glycerol and DTT.
• Shipping: The product is shipped with dry ice or equivalent.

Gene Information

• Gene Name: ISG15 ISG15 ubiquitin like modifier [ Homo sapiens (human) ]
• Official Symbol: ISG15
• Synonyms: ISG15; ISG15 ubiquitin like modifier; G1P2; IP17; UCRP; IFI15; IMD38; hUCRP; ubiquitin-like protein ISG15; interferon, alpha-inducible protein (clone IFI-15K); interferon-induced 17-kDa/15-kDa protein; interferon-stimulated protein, 15 kDa; ubiquitin cross-reactive protein
• Gene ID: 9636
• mRNA Refseq: NM_005101
• Protein Refseq: NP_005092
• MIM: 147571
• UniProt ID: P05161