Active Recombinant Human ITGA6&ITGB1 protein, His-tagged

• Cat.No.: ITGA6&ITGB1-0306H
• Product Overview: Recombinant Human ITGA6&ITGB1 protein(Phe24-Gly1012(ITGA6)&Gln21-Asp728(ITGB1)), fused with His tag, was expressed in HEK293.

Specification

• Source: HEK293
• Species: Human
• Tag: His
• Form: Lyophilized from 0.22 μm filtered solution in 50 mM Tris, 150 mM NaCl, pH7.5 with trehalose as protectant.
• Bio-activity: Immobilized Human ITGA6&ITGB1 Heterodimer Protein, His Tag&Tag Free at 5 μg/mL (100 μL/well) can bind Human Laminin 511 E8, Fc Tag with a linear range of 0.1-8 ng/mL.
• Molecular Mass: The non-reducing (NR) protein migrates as 140-150 kDa (ITGA6) and 100-118 kDa (ITGB1) respectively due to glycosylation.
• Proteinlength: Phe24-Gly1012(ITGA6)&Gln21-Asp728(ITGB1)
• Endotoxin: Less than 1.0 EU per μg by the LAL method.
• Purity: >90% as determined by SDS-PAGE.
• Storage: For long term, the product should be stored at lyophilized state at -20°C or lower.Please avoid repeated freeze-thaw cycles. This product is stable after at:
  -20°C to -70°C for 12 months in lyophilized state; -70°C for 3 months under sterile conditions after reconstitution.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.

Gene Information

• Gene Name: ITGA6 integrin, alpha 6 [ Homo sapiens ]
• Official Symbol: ITGA6
• Synonyms: ITGA6; integrin, alpha 6; integrin alpha-6; CD49f; integrin alpha6B; CD49 antigen-like family member F; VLA-6; ITGA6B; FLJ18737; DKFZp686J01244
• Gene ID: 3655
• mRNA Refseq: NM_000210
• Protein Refseq: NP_000201
• MIM: 147556
• UniProt ID: P23229

Reviews

10/12/2021

I've used this several times and their performance has been consistent and I've never had a problem.

05/22/2021

It is very suitable for crystallography research.

05/05/2021

It has good antigenicity and can induce a target immune response.

Q&As

How can I detect the expression level of ITGA6 protein?

The expression level of ITGA6 protein can be detected by immunohistochemistry, western blotting, and real-time PCR.

What is the effect of aberrant expression of ITGA6 protein on the development and metastasis of cancer?

Aberrant expression of ITGA6 protein can promote the adhesion and migration of cancer cells, thereby contributing to the development and metastasis of cancer. High expression of ITGA6 is often associated with the degree of malignancy and poor prognosis of the cancer.

What are the current challenges and controversies in the research on ITGA6 protein?

There are some challenges and controversies in the current research on ITGA6 protein, including its specific mechanism of action in different diseases, its interaction with other molecules, and the optimal treatment. In addition, more clinical data is needed to validate the reliability of ITGA6 protein as a biomarker.

What are the potential applications of ITGA6 protein in drug discovery?

Due to its critical role in a variety of diseases, ITGA6 protein may become an important target for drug discovery. Drugs targeting the ITGA6 protein may have the potential to treat diseases such as cancer, fibrotic diseases, and inflammatory diseases.

Is there a targeted therapy for the ITGA6 protein?

At present, targeted therapies targeting ITGA6 protein are still in the research stage, and there are no drugs approved for clinical use. However, some studies are exploring the use of antibodies or small molecule inhibitors to block the function of ITGA6 to treat related diseases.

Can the expression level of ITGA6 protein be used as a biomarker for disease prognosis or treatment response?

Some studies have shown that the expression level of ITGA6 protein can serve as a biomarker for the prognosis and treatment response of certain cancers. However, more studies are needed to verify its clinical application value.