Active Recombinant Human Itgb1 protein, His-tagged

• Cat.No.: Itgb1 -1624H
• Product Overview: Recombinant Human Itgb1 protein(Phe24-Gly1012|Asn28-Ser710), fused with His tag, was expressed in HEK293.

Specification

• Source: HEK293
• Species: Human
• Tag: His
• Form: Lyophilized from 0.22 μm filtered solution in 50 mM Tris,150 mM NaCl,0.2 M Arginine,pH7.5 with trehalose as protectant.
• Bio-activity: Immobilized Human Integrin alpha 6 beta 4 Protein, His Tag&Tag Free at 5 μg/mL (100 μL/well) can bind Human Laminin 511 E8, Fc Tag with a linear range of 0.8-13 ng/mL.Immobilized Human Laminin 511 E8, Fc Tag at 5 μg/mL (100 μL/well) can bind Human Integrin alpha 6 beta 4 Protein, His Tag&Tag Free with a linear range of 0.01-0.156 μg/mL.
• Molecular Mass: Human Integrin alpha 6 beta 4 Protein, His Tag&Tag Free, produced by co-expression of ITGA6 and ITGB4, has a calculated MW of 116.8 kDa (ITGA6) and 81.8 kDa (ITGB4). Subunit ITGA6 is fused with an acidic tail at the C-terminus and followed by a polyhistidine tag and subunit ITGB4 contains no tag but a basic tail at the C-terminus. The non-reducing (NR) protein migrates as 130-140 kDa (ITGA6) and 75-85 kDa (ITGB4) respectively due to glycosylation.
• Proteinlength: Phe24-Gly1012|Asn28-Ser710
• Endotoxin: Less than 1.0 EU per μg by the LAL method.
• Purity: >95% as determined by SDS-PAGE. >90% as determined by SEC-MALS.
• Storage: For long term, the product should be stored at lyophilized state at -20°C or lower.Please avoid repeated freeze-thaw cycles. This product is stable after at:
  -20°C to -70°C for 12 months in lyophilized state; -70°C for 3 months under sterile conditions after reconstitution.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.

Gene Information

• Gene Name: ITGB1 integrin, beta 1 (fibronectin receptor, beta polypeptide, antigen CD29 includes MDF2, MSK12) [ Homo sapiens ]
• Official Symbol: Itgb1
• Synonyms: ITGB1; integrin, beta 1 (fibronectin receptor, beta polypeptide, antigen CD29 includes MDF2, MSK12); FNRB, MDF2, MSK12; integrin beta-1; CD29; GPIIA; integrin VLA-4 beta subunit; very late activation protein, beta polypeptide; FNRB; MDF2; VLAB; MSK12; VLA-BETA
• Gene ID: 3688
• mRNA Refseq: NM_002211
• Protein Refseq: NP_002202
• MIM: 135630
• UniProt ID: P05556