Active Recombinant Human ITK(352-617) Protein, N-His-tagged

• Cat.No.: ITK-114H
• Product Overview: N-terminal 6His-tagged recombinant, human Itk, amino acids 352–617, expressed by baculovirus in Sf21 insect cells. Purified using Ni2+/NTA agarose.

Specification

• Description: ITK (Interleukin-2-inducible T-cell kinase) is an important member of the Tec family of non-receptor tyrosine kinases that play a central role in signaling through antigen receptors such as the T-cell receptor, B-cell receptor, and Fce.
• Source: Sf21
• Species: Human
• Tag: His
• Protein length: 352-617 aa
• Form: 2.15mg/ml of enzyme in 50mM Tris/HCl pH7.5, 300mM NaCl, 0.1mM EGTA, 0.03% Brij-35, 270mM sucrose, 1mM benzamidine, 0.2mM PMSF, 0.1% 2-mercaptoethanol. Frozen solution.
• Bio-activity: 101U/mg, where one unit of Itk activity is defined as 1nmol phosphate incorporated into 0.333mg/ml myelin basic protein (MBP) per minute at 30 centigrade with a final ATP concentration of 100μM.
• Molecular Mass: 34 kDa
• AA Sequence: 1 MSYYHHHHHH DYDIPTTENL YFQGAMGSRY GKWVIDPSEL TFVQEIGSGQ FGLVHLGYWL 61 NKDKVAIKTI REGAMSEEDF IEEAEVMMKL SHPKLVQLYG VCLEQAPICL VFEFMEHGCL 121 SDYLRTQRGL FAAETLLGMC LDVCEGMAYL EEACVIHRDL AARNCLVGEN QVIKVSDFGM 181 TRFVLDDQYT SSTGTKFPVK WASPEVFSFS RYSSKSDVWS FGVLMWEVFS EGKIPYENRS 241 NSEVVEDIST GFRLYKPRLA STHVYQIMNH CWKERPEDRP AFSRLLRQLA EIAE
• Purity: Purity 95% by SDS-PAGE and Coomassie blue staining.
• Applications: Drug Discovery & Development
• Storage: 6 months at -70 centigrade.
• Handling Advice: Rapidly thaw the vial under cold water and immediately place on ice. Aliquot unused material into prechilled micro-centrifuge tubes and immediately snap-freeze the vials in liquid nitrogen prior to re-storage at -70 centigrade.
• Use/Stability: On receipt of material store at -70 centigrade. Unopened reagent is stable for a minimum of 1 year from date of shipment when stored at recommended storage temperature. Avoid repeat freeze/thaw cycles. For maximum recovery of product, centrifuge original vial prior to removing the cap.

Gene Information

• Gene Name: ITK IL2 inducible T cell kinase [ Homo sapiens (human) ]
• Official Symbol: ITK
• Synonyms: ITK; IL2 inducible T cell kinase; EMT; LYK; LPFS1; PSCTK2
• Gene ID: 3702
• mRNA Refseq: NM_005546.4
• Protein Refseq: NP_005537.3
• MIM: 186973
• UniProt ID: Q08881