Active Recombinant Human KDR(790-end) Protein, N-His-tagged

• Cat.No.: KDR-122H
• Product Overview: N-terminal 6Histagged, recombinant, human KDR amino acids 790–end, expressed by baculovirus in Sf21insect cells. Purified using Ni2+/NTA agarose.

Specification

• Description: Two high affinity VEGF receptors, Flt-1 (VEGFR-1, activated by placental growth factor PlGF) and KDR (Kinase insert domain receptor, Flk-1/VEGFR2, activated by VEGF), have been identified. These receptors can be divided into three structural regions: seven extracellular Ig-like domains that contain the growth factor binding sites, a single polypeptide chain hydrophobic trans-membrane sequence, and intracellular cytoplasmic domains that confer the tyrosine kinase activity required for signal transduction. VEGF-B and VEGF-D homologues bind with high affinity to FLT1 (fms-related tyrosine kinase 1, vascular endothelial growth factor/vascular permeability factor receptor).
• Source: Sf21
• Species: Human
• Tag: His
• Protein length: 790-end
• Form: 0.75mg/ml of enzyme in 50mM Tris/HCl pH7.5, 300mM NaCl, 0.1mM EGTA, 0.03 % Brij-35, 270mM sucrose, 1mM benzamidine, 0.2mM PMSF, 0.1% 2-mercaptoethanol. Frozen solution.
• Bio-activity: 397U/mg, where one unit of KDR activity is defined as 1nmol phosphate incorporated into 0.33mg/ml myelin basic protein (MBP) per minute at 30 centigrade with a final ATP concentration of 100μM.
• Molecular Mass: 67.9 kDa
• AA Sequence: 1 MSYYHHHHHH DYDIPTTENL YFQGAMDPEF KGLRRLKRAN GGELKTGYLS IVMDPDELPL 61 DEHCERLPYD ASKWEFPRDR LKLGKPLGRG AFGQVIEADA FGIDKTATCR TVAVKMLKEG 121 ATHSEHRALM SELKILIHIG HHLNVVNLLG ACTKPGGPLM VIVEFCKFGN LSTYLRSKRN 181 EFVPYKTKGA RFRQGKDYVG AIPVDLKRRL DSITSSQSSA SSGFVEEKSL SDVEEEEAPE 241 DLYKDFLTLE HLICYSFQVA KGMEFLASRK CIHRDLAARN ILLSEKNVVK ICDFGLARDI 301 YKDPDYVRKG DARLPLKWMA PETIFDRVYT IQSDVWSFGV LLWEIFSLGA SPYPGVKIDE 361 EFCRRLKEGT RMRAPDYTTP EMYQTMLDCW HGEPSQRPTF SELVEHLGNL LQANAQQDGK 421 DYIVLPISET LSMEEDSGLS LPTSPVSCME EEEVCDPKFH YDNTAGISQY LQNSKRKSRP 481 VSVKTFEDIP LEEPEVKVIP DDNQTDSGMV LASEELKTLE DRTKLSPSFG GMVPSKSRES 541 VASEGSNQTS GYQSGYHSDD TDTTVYSSEE AELLKLIEIG VQTGSTAQIL QPDSGTTLSS 601 PPV
• Purity: Purity 85% by SDS-PAGE and Coomassie blue staining.
• Applications: Drug Discovery & Development
• Storage: 6 months at -70 centigrade.
• Handling Advice: Rapidly thaw the vial under cold water and immediately place on ice. Aliquot unused material into prechilled micro-centrifuge tubes and immediately snap-freeze the vials in liquid nitrogen prior to re-storage at -70 centigrade.
• Use/Stability: On receipt of material store at -70 centigrade. Unopened reagent is stable for a minimum of 1 year from date of shipment when stored at recommended storage temperature. Avoid repeat freeze/thaw cycles. For maximum recovery of product, centrifuge original vial prior to removing the cap.

Gene Information

• Gene Name: KDR kinase insert domain receptor [ Homo sapiens (human) ]
• Official Symbol: KDR
• Synonyms: KDR; kinase insert domain receptor; FLK1; CD309; VEGFR; VEGFR2
• Gene ID: 3791
• mRNA Refseq: NM_002253.4
• Protein Refseq: NP_002244.1
• MIM: 191306
• UniProt ID: P35968