Active Recombinant Human KITLG (26-189aa), Met tagged

• Cat.No.: KITLG-036E
• Product Overview: Recombinant Human KITLG (Glu26 ­ Ala189, with an N­terminal Met) was expressed in E. coli and produced using non­animal reagent in an animal­free laboratory.

Specification

• Cat. No.: KITLG-036E
• Description: Stem cell factor (SCF), also known as c­kit ligand (KL), mast cell growth factor (MGF), and steel factor (SLF), is a widely expressed 28 ­ 40 kDa type I transmembrane glycoprotein. It promotes the survival, differentiation, and mobilization of multiple cell types including myeloid, erythroid, megakaryocytic, lymphoid, germ cell, and melanocyte progenitors. SCF is a primary growth and activation factor for mast cells and eosinophils. SCF assists in the recovery of cardiac function following myocardial infarction by increasing the number of cardiomyocytes and vascular channels.
• Source: E. coli
• N­terminal Sequence Analysi: Met
• Molecular Mass: 18.6 kDa
• Activity: Measured in a cell proliferation assay using TF­1 human erythroleukemic cells. The ED50 for this effect is typically 1 ­ 5 ng/mL.
• Endotoxin Leve: <1.0 EU per 1 μg of the protein by the LAL method.
• Purity: >97%, by SDS­PAGE under reducing conditions and visualized by silver stain.
• Formulation: Lyophilized from a 0.2 μm filtered solution in Acetonitrile and TFA. See Certificate of Analysis for details.
• Reconstitution: Reconstitute at 0.2 mg/mL in sterile PBS.
• Shipping: The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
• Storage: Use a manual defrost freezer and avoid repeated freeze­thaw cycles. 1. 12 months from date of receipt, ­20 to ­70 °C as supplied. 2. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 3. 3 months, ­20 to ­70 °C under sterile conditions after reconstitution.
• Unitprot ID: P21583

Gene Information

• Gene Name: KITLG KIT ligand [ Homo sapiens ]
• Official Symbol: KITLG
• Synonyms: KITLG; KIT ligand; SF; MGF; SCF; FPH2; KL-1; Kitl; SHEP7; kit-ligand; DKFZp686F2250; c-Kit ligand; steel factor; stem cell factor; mast cell growth factor; familial progressive hyperpigmentation 2
• Gene ID: 4254
• mRNA Refseq: NM_003994
• Protein Refseq: NP_003985
• MIM: 184745
• Chromosome Location: 12q22
• Pathway: Cytokine-cytokine receptor interaction; Hematopoietic cell lineage; Kit Receptor Signaling Pathway; Melanogenesis; Pathways in cancer
• Function: cytokine activity; growth factor activity; protein binding; stem cell factor receptor binding