Active Recombinant Human KITLG Protein (Glu26-Ala189), GMP Grade, Animal-Free
Cat.No. : | KITLG-15HG |
Product Overview : | GMP recombinant human SCF protein (Glu26-Ala189) with an N-terminal Met was expressed in E. coli, produced using non-animal reagents in an Animal-Free laboratory and manufactured and tested under cGMP guidelines. |
- Specification
- Gene Information
- Related Products
Description : | This gene encodes the ligand of the tyrosine-kinase receptor encoded by the KIT locus. This ligand is a pleiotropic factor that acts in utero in germ cell and neural cell development, and hematopoiesis, all believed to reflect a role in cell migration. In adults, it functions pleiotropically, while mostly noted for its continued requirement in hematopoiesis. Two transcript variants encoding different isoforms have been found for this gene. |
Source : | E. coli |
Species : | Human |
Bio-activity : | Measured in a cell proliferation assay using TF-1 human erythroleukemic cells. The ED50 for this effect is 1-5 ng/mL. The specific activity of recombinant human SCF is >1.0 x 106 units/mg, which is calibrated against the human SCF reference standard. |
Molecular Mass : | Predicted Molecular Mass: 19 kDa SDS-PAGE: 17-18 kDa, reducing conditions |
Protein Length : | Glu26-Ala189 |
N-terminal Sequence Analysis : | Met-Glu26-Gly-Ile-(Cys)-Arg-Asn-Arg-Val-Thr |
Endotoxin : | <0.10 EU/ μg of the protein by the LAL method. |
Purity : | >97%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining. |
Storage : | Use a manual defrost freezer and avoid repeated freeze-thaw cycles. A minimum of 12 months when stored at ≤ -20 centigrade as supplied. Refer to lot specific COA for the Use by Date. 1 month, 2 to 8 centigrade under sterile conditions after reconstitution. 3 months, ≤ -20 centigrade under sterile conditions after reconstitution. |
Storage Buffer : | Lyophilized from a 0.2 μm filtered solution in PBS. |
Reconstitution : | Reconstitute at 100 μg/mL in PBS. |
Shipping : | The product is shipped with polar packs. |
Gene Name : | KITLG KIT ligand [ Homo sapiens ] |
Official Symbol : | KITLG |
Synonyms : | KITLG; KIT ligand; MGF; kit ligand; familial progressive hyperpigmentation 2; FPH2; Kitl; KL 1; mast cell growth factor; SCF; SF; steel factor; stem cell factor; c-Kit ligand; KL-1; SHEP7; kit-ligand; DKFZp686F2250; |
Gene ID : | 4254 |
mRNA Refseq : | NM_000899 |
Protein Refseq : | NP_000890 |
MIM : | 184745 |
UniProt ID : | P21583 |
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◆ Lysates | ||
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Related Gene
For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.
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