Active Recombinant Human LIMK2(2-end) Protein, N-His-tagged

• Cat.No.: LIMK2-125H
• Product Overview: N-terminal 6His-tagged, recombinant, human LIMK2 amino acids 2-end expressed by baculovirus in Sf21 insect cells. Purified using immobilized metal affinity chromatography.

Specification

• Description: LIMK2, LIM domain kinase 2, is a serine/threonine-protein kinase that plays an essential role in the regulation of actin filament dynamics. This kinase has also been implicated in brain development.
• Source: Sf21
• Species: Human
• Tag: His
• Protein length: 2-end
• Form: 0.65 mg/ml of enzyme in 50 mM Tris/HCl pH 7.5, 300 mM NaCl, 0.1 mM EGTA, 0.03% Brij-35, 270 mM sucrose, 1 mM benzamidine, 0.2 mM PMSF, 0.1% 2-mercaptoethanol. Frozen solution.
• Bio-activity: 15 U/mg, where one unit of LIMK2 activity is defined as 1 nmol phosphate incorporated into 0.96 mg/ml cofilin 1 per minute at 30 centigrade with a final ATP concentration of 100 μM.
• Molecular Mass: 76 kDa
• Purity: 82% by SDS-PAGE and Coomassie blue staining.
• Applications: Drug Discovery & Development
• Storage: 1 year at -70 centigrade.
• Handling Advice: Rapidly thaw the vial under cold water and immediately place on ice. Aliquot unused material into prechilled micro-centrifuge tubes and immediately snap-freeze the vials in liquid nitrogen prior to re-storage at -70 centigrade.
• Use/Stability: On receipt of material store at -70 centigrade. Unopened reagent is stable for a minimum of 1 year from date of shipment when stored at recommended storage temperature. Avoid repeat freeze/thaw cycles. For maximum recovery of product, centrifuge original vial prior to removing the cap.

Gene Information

• Gene Name: LIMK2 LIM domain kinase 2 [ Homo sapiens (human) ]
• Official Symbol: LIMK2
• Synonyms: LIMK2; LIM domain kinase 2; LIM Domain Kinase 2; LIMK-2
• Gene ID: 3985
• mRNA Refseq: NM_005569.4
• Protein Refseq: NP_005560.1
• MIM: 601988
• UniProt ID: P53671