Active Recombinant Human LTK(450-end) Protein, N-GST-tagged

• Cat.No.: LTK-128H
• Product Overview: N-terminal GST-tagged, recombinant, human LTK amino acids 450–end expressed by baculovirus in Sf21 insect cells. Purified using glutathione agarose followed by gel filtration.

Specification

• Description: LTK is a member of the ros/insulin receptor family of tyrosine kinases. Tyrosine-specific phosphorylation of proteins is a key to the control of diverse pathways leading to cell growth and differentiation. Two alternatively spliced transcript variants encoding different isoforms have been described for this gene.
• Source: Sf21
• Species: Human
• Tag: GST
• Protein length: 450-end
• Form: 0.53mg/ml of enzyme in 50mM Tris/HCl pH7.5, 300mM NaCl, 0.1mM EGTA, 0.03% Brij-35, 270mM sucrose, 1mM benzamidine, 0.2mM PMSF, 0.1% βmercaptoethanol. Frozen solution.
• Bio-activity: 1779U/mg, where one unit of LTK, active activity is defined as 1nmol phosphate incorporated into 250μM (GEEPLYWSFPAKKK) per minute at 30 centigrade with a final ATP concentration of 100μM.
• Molecular Mass: 74 kDa
• AA Sequence: 1 MSPILGYWKI KGLVQPTRLL LEYLEEKYEE HLYERDEGDK WRNKKFELGL EFPNLPYYID 61 GDVKLTQSMA IIRYIADKHN MLGGCPKERA EISMLEGAVL DIRYGVSRIA YSKDFETLKV 121 DFLSKLPEML KMFEDRLCHK TYLNGDHVTH PDFMLYDALD VVLYMDPMCL DAFPKLVCFK 181 KRIEAIPQID KYLKSSKYIA WPLQGWQATF GGGDHPPKSD LEVLFQGPEF KGLRRHKQKK 241 WQGLQEMRLP SPELELSKLR TSAIRTAPNP YYCQVGLGPA QSWPLPPGVT EVSPANVTLL 301 RALGHGAFGE VYEGLVIGLP GDSSPLQVAI KTLPELCSPQ DELDFLMEAL IISKFRHQNI 361 VRCVGLSLRA TPRLILLELM SGGDMKSFLR HSRPHLGQPS PLVMRDLLQL AQDIAQGCHY 421 LEENHFIHRD IAARNCLLSC AGPSRVAKIG DFGMARDIYR ASYYRRGDRA LLPVKWMPPE 481 AFLEGIFTSK TDSWSFGVLL WEIFSLGYMP YPGRTNQEVL DFVVGGGRMD PPRGCPGPVY 541 RIMTQCWQHE PELRPSFASI LERLQYCTQD PDVLNSLLPM ELGPTPEEEG TSGLGNRSLE 601 CLRPPQPQEL SPEKLKSWGG SPLGPWLSSG LKPLKSRGLQ PQNLWNPTYR S
• Purity: Purity 94% by SDS-PAGE and Coomassie blue staining.
• Applications: Drug Discovery & Development
• Storage: 1 year at -70 centigrade.
• Handling Advice: Rapidly thaw the vial under cold water and immediately place on ice. Aliquot unused material into prechilled micro-centrifuge tubes and immediately snap-freeze the vials in liquid nitrogen prior to re-storage at -70 centigrade.
• Use/Stability: On receipt of material store at -70 centigrade. Unopened reagent is stable for a minimum of 1 year from date of shipment when stored at recommended storage temperature. Avoid repeat freeze/thaw cycles. For maximum recovery of product, centrifuge original vial prior to removing the cap.

Gene Information

• Gene Name: LTK leukocyte receptor tyrosine kinase [ Homo sapiens (human) ]
• Official Symbol: LTK
• Synonyms: LTK; leukocyte receptor tyrosine kinase; TYK1
• Gene ID: 4058
• mRNA Refseq: NM_002344.6
• Protein Refseq: NP_002335.2
• MIM: 151520
• UniProt ID: P29376