Active Recombinant Human MAP2K6(S599D, T603D)(4-end) Mutation Protein, N-MBP-tagged

• Cat.No.: MAP2K6-147H
• Product Overview: N-terminal MBP tagged, recombinant human MKK6/SKK3 residues 4–end containing S599D and T603D mutations. Expressed in E.coli cells. Purified using amylose agarose.

Specification

• Description: MKK6/SKK3 Protein is a member of the dual specificity protein kinase family, which functions as a mitogen-activated protein (MAP) Kinase kinase. MAP kinases, also known as extracellular signal-regulated kinases (ERKs), act as an integration point for multiple biochemical signals. This protein phosphorylates and activates p38 MAP kinase in response to inflammatory cytokines or environmental stress. As an essential component of p38 MAP kinase mediated signal transduction pathway, this gene is involved in many cellular processes such as stress induced cell cycle arrest, transcription activation and apoptosis.
• Source: E. coli
• Species: Human
• Tag: MBP
• Protein length: 4-end
• Form: 3.74mg/ml of enzyme in 50mM Tris/HCl pH7.5, 150mM NaCl, 0.03% Brij-35, 0.1mM EGTA, 0.2mM PMSF, 1mM benzamidine, 0.1% 2- mercaptoethanol, 270mM sucrose. Frozen solution.
• Bio-activity: 1431U/mg, where one unit of MKK6/SKK3 activity is defined as the amount of MKK6/SKK3 which activates 2μM p38α/SAPK2a, unactive by 1 Unit per minute using 100μM ATP at 30 centigrade. One unit of p38α/SAPK2a activity is defined as 1nmole of phosphate incorporated into 0.33mg/ml myelin basic protein per minute at 30 centigrade with a final ATP concentration of 100μM.
• Molecular Mass: 80.6 kDa
• AA Sequence: 1 MKTEEGKLVI WINGDKGYNG LAEVGKKFEK DTGIKVTVEH PDKLEEKFPQ VAATGDGPDI 61 IFWAHDRFGG YAQSGLLAEI TPDKAFQDKL YPFTWDAVRY NGKLIAYPIA VEALSLIYNK 121 DLLPNPPKTW EEIPALDKEL KAKGKSALMF NLQEPYFTWP LIAADGGYAF KYENGKYDIK 181 DVGVDNAGAK AGLTFLVDLI KNKHMNADTD YSIAEAAFNK GETAMTINGP WAWSNIDTSK 241 VNYGVTVLPT FKGQPSKPFV GVLSAGINAA SPNKELAKEF LENYLLTDEG LEAVNKDKPL 301 GAVALKSYEE ELAKDPRIAA TMENAQKGEI MPNIPQMSAF WYAVRTAVIN AASGRQTVDE 361 ALKDAQTNSS SNNNNNNNNN NLGIEGRISE FGSSRSKGKK RNPGLKIPKE AFEQPQTSST 421 PPRDLDSKAC ISIGNQNFEV KADDLEPIME LGRGAYGVVE KMRHVPSGQI MAVKRIRATV 481 NSQEQKRLLM DLDISMRTVD CPFTVTFYGA LFREGDVWIC MELMDTSLDK FYKQVIDKGQ 541 TIPEDILGKI AVSIVKALEH LHSKLSVIHR DVKPSNVLIN ALGQVKMCDF GISGYLVDDV 601 AKDIDAGCKP YMAPERINPE LNQKGYSVKS DIWSLGITMI ELAILRFPYD SWGTPFQQLK 661 QVVEEPSPQL PADKFSAEFV DFTSQCLKKN SKERPTYPEL MQHPFFTLHE SKGTDVASFV 721 KLILGD
• Purity: Purity 88.4% by SDS-PAGE and Coomassie staining.
• Applications: Drug Discovery & Development
• Storage: 6 months at -70 centigrade.
• Handling Advice: Rapidly thaw the vial under cold water and immediately place on ice. Aliquot unused material into prechilled micro-centrifuge tubes and immediately snap-freeze the vials in liquid nitrogen prior to re-storage at -70 centigrade.
• Use/Stability: On receipt of material store at -70 centigrade. Unopened reagent is stable for a minimum of 1 year from date of shipment when stored at recommended storage temperature. Avoid repeat freeze/thaw cycles. For maximum recovery of product, centrifuge original vial prior to removing the cap.

Gene Information

• Gene Name: MAP2K6 mitogen-activated protein kinase kinase 6 [ Homo sapiens (human) ]
• Official Symbol: MAP2K6
• Synonyms: MAP2K6; mitogen-activated protein kinase kinase 6; MEK6; MKK6; MAPKK6; PRKMK6; SAPKK3; SAPKK-3
• Gene ID: 5608
• mRNA Refseq: NM_002758.4
• Protein Refseq: NP_002749.2
• MIM: 601254
• UniProt ID: P52564