Active Recombinant Human MAP3K10(1-449) Protein, N-His-tagged

• Cat.No.: MAP3K10-153H
• Product Overview: N-terminal 6Histagged, recombinant, human MLK2, amino acids 1-449 expressed by baculovirus in Sf21 insect cells. Purified using immobilized metal affinity chromatography.

Specification

• Description: MLK2 is a member of the serine/threonine kinase family. This kinase has been shown to activate MAPK8/JNK and MKK4/SEK1, and this kinase itself can be phosphorylated, and thus activated by JNK kinases. This kinase functions preferentially on the JNK signaling pathway, and is reported to be involved in nerve growth factor (NGF) induced neuronal apoptosis.
• Source: Sf21
• Species: Human
• Tag: His
• Protein length: 1-449 aa
• Form: 0.65mg/ml of enzyme in 50mM Tris/HCl pH7.5, 300mM NaCl, 0.1mM EGTA, 0.03% Brij-35, 270mM sucrose, 1mM benzamidine, 0.2mM PMSF, 0.1% 2-mercaptoethanol. Frozen solution.
• Bio-activity: 532U/mg, where one unit of MLK2 active activity is defined as 1nmol phosphate incorporated into 0.33mg/ml myelin basic protein per minute at 30 centigrade with a final ATP concentration of 100μM.
• Molecular Mass: 54 kDa
• AA Sequence: 1 MSYYHHHHHH DYDIPTTENL YFQGAMDPEF MEEEEGAVAK EWGTTPAGPV WTAVFDYEAA 61 GDEELTLRRG DRVQVLSQDC AVSGDEGWWT GQLPSGRVGV FPSNYVAPGA PAAPAGLQLP 121 QEIPFHELQL EEIIGVGGFG KVYRALWRGE EVAVKAARLD PEKDPAVTAE QVCQEARLFG 181 ALQHPNIIAL RGACLNPPHL CLVMEYARGG ALSRVLAGRR VPPHVLVNWA VQVARGMNYL 241 HNDAPVPIIH RDLKSINILI LEAIENHNLA DTVLKITDFG LAREWHKTTK MSAAGTYAWM 301 APEVIRLSLF SKSSDVWSFG VLLWELLTGE VPYREIDALA VAYGVAMNKL TLPIPSTCPE 361 PFARLLEECW DPDPHGRPDF GSILKRLEVI EQSALFQMPL ESFHSLQEDW KLEIQHMFDD 421 LRTKEKELRS REEELLRAAQ EQRFQEEQLR RREQELAERE MDIVERELHL LMCQLSQEK
• Purity: Purity 82% by SDS-PAGE and Coomassie blue staining.
• Applications: Drug Discovery & Development
• Storage: 1 year at -70 centigrade.
• Handling Advice: Rapidly thaw the vial under cold water and immediately place on ice. Aliquot unused material into prechilled micro-centrifuge tubes and immediately snap-freeze the vials in liquid nitrogen prior to re-storage at -70 centigrade.
• Use/Stability: On receipt of material store at -70 centigrade. Unopened reagent is stable for a minimum of 1 year from date of shipment when stored at recommended storage temperature. Avoid repeat freeze/thaw cycles. For maximum recovery of product, centrifuge original vial prior to removing the cap.

Gene Information

• Gene Name: MAP3K10 mitogen-activated protein kinase kinase kinase 10 [ Homo sapiens (human) ]
• Official Symbol: MAP3K10
• Synonyms: MAP3K10; mitogen-activated protein kinase kinase kinase 10; MST; MLK2; MEKK10
• Gene ID: 4294
• mRNA Refseq: NM_002446.4
• Protein Refseq: NP_002437.2
• MIM: 600137
• UniProt ID: Q02779