Active Recombinant Human MARK1(2-end) Protein, N-His-tagged

• Cat.No.: MARK1-135H
• Product Overview: N-terminal 6Histagged, recombinant, human MARK1, amino acids 2–end, expressed by baculovirus in Sf21 cells. Purified using Ni2+/NTA agarose.

Specification

• Description: MARK (MAP/microtubule affinity-regulating kinase) phosphorylates the microtubule-associated proteins tau, MAP2, and MAP4 on their microtubule-binding domain, causing their dissociation from microtubules and increased microtubule dynamics. Overexpression of MARK1 or MARK2 in cells leads to hyperphosphorylation of MAPs and to disruption of the microtubule array, resulting in morphological changes and cell death.
• Source: Sf21
• Species: Human
• Tag: His
• Protein length: 2-end
• Form: 0.788mg/ml of enzyme in 50mM Tris/HCl pH7.5, 300mM NaCl, 0.1mM EGTA, 0.03% Brij-35, 270mM sucrose, 1mM benzamidine, 0.2mM PMSF, 0.1% 2-mercaptoethanol. Frozen solution.
• Bio-activity: 1304U/mg, where one unit of MARK1, active activity is defined as 1nmol phosphate incorporated into 100μM CHKtide (KKKVSRSGLYRSPSMPENLNRPR) per minute at 30 centigrade with a final ATP concentration of 100μM.
• Molecular Mass: 92.7 kDa
• AA Sequence: 1 MSYYHHHHHH DYDIPTTENL YFQGAMDPEF SARTPLPTVN ERDTENHTSV DGYTEPHIQP 61 TKSSSRQNIP RCRNSITSAT DEQPHIGNYR LQKTIGKGNF AKVKLARHVL TGREVAVKII 121 DKTQLNPTSL QKLFREVRIM KILNHPNIVK LFEVIETEKT LYLVMEYASG GEVFDYLVAH 181 GRMKEKEARA KFRQIVSAVQ YCHQKYIVHR DLKAENLLLD GDMNIKIADF GFSNEFTVGN 241 KLDTFCGSPP YAAPELFQGK KYDGPEVDVW SLGVILYTLV SGSLPFDGQN LKELRERVLR 301 GKYRIPFYMS TDCENLLKKL LVLNPIKRGS LEQIMKDRWM NVGHEEEELK PYTEPDPDFN 361 DTKRIDIMVT MGFARDEIND ALINQKYDEV MATYILLGRK PPEFEGGESL SSGNLCQRSR 421 PSSDLNNSTL QSPAHLKVQR SISANQKQRR FSDHAGPSIP PAVSYTKRPQ ANSVESEQKE 481 EWDKDVARKL GSTTVGSKSE MTASPLVGPE RKKSSTIPSN NVYSGGSMAR RNTYVCERTT 541 DRYVALQNGK DSSLTEMSVS SISSAGSSVA SAVPSARPRH QKSMSTSGHP IKVTLPTIKD 601 GSEAYRPGTT QRVPAASPSA HSISTATPDR TRFPRGSSSR STFHGEQLRE RRSVAYNGPP 661 ASPSHETGAF AHARRGTSTG IISKITSKFV RRDPSEGEAS GRTDTSRSTS GEPKERDKEE 721 GKDSKPRSLR FTWSMKTTSS MDPNDMMREI RKVLDANNCD YEQKERFLLF CVHGDARQDS 781 LVQWEMEVCK LPRLSLNGVR FKRISGTSIA FKNIASKIAN ELKL
• Purity: Purity 56.9% by SDSPAGE and Coomassie blue staining.
• Applications: Drug Discovery & Development
• Storage: 6 months at -70 centigrade.
• Handling Advice: Rapidly thaw the vial under cold water and immediately place on ice. Aliquot unused material into prechilled micro-centrifuge tubes and immediately snap-freeze the vials in liquid nitrogen prior to re-storage at -70 centigrade.
• Use/Stability: On receipt of material store at -70 centigrade. Unopened reagent is stable for a minimum of 1 year from date of shipment when stored at recommended storage temperature. Avoid repeat freeze/thaw cycles. For maximum recovery of product, centrifuge original vial prior to removing the cap.

Gene Information

• Gene Name: MARK1 microtubule affinity regulating kinase 1 [ Homo sapiens (human) ]
• Official Symbol: MARK1
• Synonyms: MARK1; microtubule affinity regulating kinase 1; MARK; Par1c; Par-1c
• Gene ID: 4139
• mRNA Refseq: NM_018650.5
• Protein Refseq: NP_061120.3
• MIM: 606511
• UniProt ID: Q9P0L2