| Description : |
MET tyrosine kinase is a high-affinity receptor for hepatocyte growth factor/scatter factor. Signaling via this receptor-ligand pair has been shown to affect a wide range of biological activities, including angiogenesis, cellular motility, growth, invasion, and morphogenic differentiation. Mutations in the MET tyrosine kinase receptor have been identified in both hereditary and sporadic forms of papillary renal carcinoma and implicated in tumor growth, invasion, and metastasis. |
| Source : |
Sf21 |
| Species : |
Human |
| Tag : |
His |
| Protein length : |
974-end |
| Form : |
0.669mg/ml of enzyme in 50mM Tris/HCl pH7.5, 300mM NaCl, 0.1mM EGTA, 0.03% Brij-35, 270mM sucrose, 1mM benzamidine, 0.2mM PMSF, 0.1% 2-mercaptoethanol. Frozen solution. |
| Bio-activity : |
741U/mg, where one unit of Met (M1268T), active activity is defined as 1nmol phosphate incorporated into 250μM (KKKGQEEEYVFIE) per minute at 30 centigrade with a final ATP concentration of 100μM. |
| Molecular Mass : |
50.1 kDa |
| AA Sequence : |
1 MHHHHHHKKR KQIKDLGSEL VRYDARVHTP HLDRLVSARS VSPTTEMVSN ESVDYRATFP 61 EDQFPNSSQN GSCRQVQYPL TDMSPILTSG DSDISSPLLQ NTVHIDLSAL NPELVQAVQH 121 VVIGPSSLIV HFNEVIGRGH FGCVYHGTLL DNDGKKIHCA VKSLNRITDI GEVSQFLTEG 181 IIMKDFSHPN VLSLLGICLR SEGSPLVVLP YMKHGDLRNF IRNETHNPTV KDLIGFGLQV 241 AKGMKYLASK KFVHRDLAAR NCMLDEKFTV KVADFGLARD MYDKEYYSVH NKTGAKLPVK 301 WTALESLQTQ KFTTKSDVWS FGVLLWELMT RGAPPYPDVN TFDITVYLLQ GRRLLQPEYC 361 PDPLYEVMLK CWHPKAEMRP SFSELVSRIS AIFSTFIGEH YVHVNATYVN VKCVAPYPSL 421 LSSEDNADDE VDTRPASFWE TS |
| Purity : |
Purity 71% by SDS-PAGE and Coomassie blue staining. |
| Applications : |
Drug Discovery & Development |
| Storage : |
6 months at -70 centigrade. |
| Handling Advice : |
Rapidly thaw the vial under cold water and immediately place on ice. Aliquot unused material into prechilled micro-centrifuge tubes and immediately snap-freeze the vials in liquid nitrogen prior to re-storage at -70 centigrade. |
| Use/Stability : |
On receipt of material store at -70 centigrade. Unopened reagent is stable for a minimum of 1 year from date of shipment when stored at recommended storage temperature. Avoid repeat freeze/thaw cycles. For maximum recovery of product, centrifuge original vial prior to removing the cap. |
