Active Recombinant Human MINK1(1-310) Protein, N-His-tagged

• Cat.No.: MINK1-144H
• Product Overview: N-terminal 6Histagged, recombinant, human MINK amino acids 1–310, expressed by baculovirus in Sf21 insect cells. Purified using Ni2+/NTA agarose.

Specification

• Description: MINK protein is a serine/threonine kinase belonging to the germinal center kinase (GCK) family. The protein is structurally similar to the kinases that are related to NIK and may belong to a distinct subfamily of NIK-related kinases within the GCK family. Studies of the mouse homolog indicate an up-regulation of expression in the course of postnatal mouse cerebral development and activation of the cJun N-terminal kinase (JNK) and the p38 pathways. Alternative splicing occurs at this locus and four transcript variants encoding distinct isoforms have been identified.
• Source: Sf21
• Species: Human
• Tag: His
• Protein length: 1-310 aa
• Form: 1.107mg/ml of enzyme in 50mM Tris/HCl pH7.5, 300mM NaCl, 0.1mM EGTA, 0.03% Brij-35, 270mM sucrose, 1mM benzamidine, 0.2mM PMSF, 0.1% 2-mercaptoethanol. Frozen solution.
• Bio-activity: 594U/mg, where one unit of MINK activity is defined as 1nmol phosphate incorporated into 0.33mg/ml myelin basic protein per minute at 30 centigrade with a final ATP concentration of 100μM.
• Molecular Mass: 38.7 kDa
• AA Sequence: 1 MSYYHHHHHH DYDIPTTENL YFQGAMGSMG DPAPARSLDD IDLSALRDPA GIFELVEVVG 61 NGTYGQVYKG RHVKTGQLAA IKVMDVTEDE EEEIKQEINM LKKYSHHRNI ATYYGAFIKK 121 SPPGNDDQLW LVMEFCGAGS VTDLVKNTKG NALKEDCIAY ICREILRGLA HLHAHKVIHR 181 DIKGQNVLLT ENAEVKLVDF GVSAQLDRTV GRRNTFIGTP YWMAPEVIAC DENPDATYDY 241 RSDIWSLGIT AIEMAEGAPP LCDMHPMRAL FLIPRNPPPR LKSKKWSKKF IDFIDTCLIK 301 TYLSRPPTEQ LLKFPFIRDQ PTERQVRIQL KDHIDRSR
• Purity: Purity 86.7% by SDS-PAGE and Coomassie blue staining.
• Applications: Drug Discovery & Development
• Storage: 6 months at -70 centigrade.
• Handling Advice: Rapidly thaw the vial under cold water and immediately place on ice. Aliquot unused material into prechilled micro-centrifuge tubes and immediately snap-freeze the vials in liquid nitrogen prior to re-storage at -70 centigrade.
• Use/Stability: On receipt of material store at -70 centigrade. Unopened reagent is stable for a minimum of 1 year from date of shipment when stored at recommended storage temperature. Avoid repeat freeze/thaw cycles. For maximum recovery of product, centrifuge original vial prior to removing the cap.

Gene Information

• Gene Name: MINK1 misshapen like kinase 1 [ Homo sapiens (human) ]
• Official Symbol: MINK1
• Synonyms: MINK1; misshapen like kinase 1; B55; ZC3; MINK; YSK2; MAP4K6; MEKKK 6
• Gene ID: 50488
• mRNA Refseq: NM_015716.5
• Protein Refseq: NP_056531.1
• MIM: 609426
• UniProt ID: Q8N4C8