| Description : |
This gene encodes a member of the peptidase M10 family of matrix metalloproteinases (MMPs). Proteins in this family are involved in the breakdown of extracellular matrix in normal physiological processes, such as embryonic development, reproduction, and tissue remodeling, as well as in disease processes, such as arthritis and metastasis. The encoded preproprotein is proteolytically processed to generate the mature protease. This secreted protease breaks down proteoglycans, fibronectin, elastin and casein and differs from most MMP family members in that it lacks a conserved C-terminal hemopexin domain. The enzyme is involved in wound healing, and studies in mice suggest that it regulates the activity of defensins in intestinal mucosa. The gene is part of a cluster of MMP genes on chromosome 11. This gene exhibits elevated expression levels in multiple human cancers. |
| Source : |
Mouse myeloma cell line NS0 |
| Species : |
Human |
| Bio-activity : |
Measured by its ability to cleave the fluorogenic peptide substrate, Mca-PLGL-Dpa-AR-NH2. The specific activity is >600 pmol/min/µg, as measured under the described conditions. |
| Molecular Mass : |
28 kDa & 20 kDa (Leu18-T198) |
| N-terminal Sequence Analysis : |
Leu18 & Thr199 |
| Endotoxin : |
<1.0 EU per 1 μg of the protein by the LAL method. |
| Purity : |
>90%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining. |
| Storage : |
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
6 months from date of receipt, -70 °C as supplied.
3 months, -70 °C under sterile conditions after opening. |
| Storage Buffer : |
Supplied as a 0.2 μm filtered solution in MES, NaCl and CaCl2. |
| Shipping : |
The product is shipped with dry ice or equivalent. Upon receipt, store it immediately at the temperature recommended below. |
| SDS-PAGE : |
28 kDa (major) & 20 kDa (minor), reducing conditions |
| Structure / Form : |
Pro form |
