| Description : |
MST1R, encoded by the human Ron and the mouse Stk, is one of a small family of receptor tyrosine kinases (RTKs) that also includes human Met (the receptor for hepatocyte growth factor) and chicken Sea (1, 2). This family of receptors is synthesized as a single-chain precursors that are cleaved into a mature disulfide-linked heterodimers composed of an extracellular alpha chain and a membrane spanning beta chain with intrinsic tyrosine kinase activity. Biologically active ligands (MSP and HGF) for this family of receptors are also disulfide-linked alpha -beta heterodimers. Human MSPR cDNA encodes a 1400 amino acid (aa) residue precursor protein with a 24 aa signal peptide, a 285 aa residue alpha chain (Glu25-Arg309) and a 1091 aa residue transmembrane beta chain (Gly310-Thr1400). The extracellular domain of MSPR is comprised of an N-terminal sema domain, a PSI (plexin semaphorins integrins) domain, followed by four immunoglobulin-like folds shared by plexins and transcription factors (3). The soluble sema domain binds MSP and inhibits the MSPR-dependent signaling pathways. MSP receptor is expressed in multiple tissues including specific areas of the central and peripheral nervous systems, epithelial cells along the digestive tract, skin and lung, and in subpopulations of the mononuclear phagocyte lineage (1, 2). Although free MSP alpha or beta chains have been shown to bind MSPR, only the heterodimeric MSP can induce receptor activation and cause biological activity (4, 5). MSPR associates with other transmembrane molecules including integrins, cadherins and other cytokine receptors. Transactivation and signaling crosstalk between MSPR and its associated transmembrane receptors have been demonstrated (6-8). Human MSPR shares 72% amino acid sequence identity with mouse MSPR. |
| Source : |
HEK293 cells |
| Species : |
Human |
| Tag : |
His/Avi |
| Predicted N Terminal : |
Glu25 ( alpha chain) and Gly310 ( beta chain) |
| Form : |
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. |
| Bio-activity : |
Measured by its binding ability in a functional ELISA. When Recombinant Human MST1 (Cys672Ala) is immobilized at 0.5 μg/mL, 100 μL/well, the concentration of Biotinylated Recombinant Human MST1 His-tag Avi-tag that produces 50% of the optimal binding response is approximately 40‑240 ng/mL. |
| Molecular Mass : |
34-42 kDa & 64-78 kDa, under reducing conditions |
| Protein length : |
Glu25-Leu571 |
| Endotoxin : |
<0.10 EU per 1 μg of the protein by the LAL method. |
| Purity : |
>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining. |
| Applications : |
Bioactivity |
| Storage : |
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.12 months from date of receipt, -20 to -70 °C as supplied.1 month, 2 to 8 °C under sterile conditions after reconstitution.3 months, -20 to -70 °C under sterile conditions after reconstitution. |
| Reconstitution : |
Reconstitute at 200 μg/mL in PBS. |
