Active Recombinant Human MUC16 protein, Fc-Avi-tagged, Biotinylated

• Cat.No.: MUC16-2979H
• Product Overview: Biotinylated Recombinant Human MUC16 protein(Gly 12660-Met 12923), fused with Fc and Avi tag, was expressed in HEK293.

Specification

• Source: HEK293
• Species: Human
• Tag: Fc-Avi
• Form: Lyophilized from 0.22 μm filtered solution in Tris with Glycine, Arginine and NaCl, pH7.5. Normally trehalose is added as protectant before lyophilization.
• Bio-activity: Immobilized Human Mesothelin, His Tag at 10 μg/mL (100 μL/well) can bind Biotinylated Human CA125, Fc,Avitag with a linear range of 2-78 ng/mL.
• Molecular Mass: This protein carries a human IgG1 Fc tag at the C-terminus, followed by an Avi tag (Avitag™).The protein has a calculated MW of 56.9 kDa. The protein migrates as 75-100 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
• Protein Length: Gly 12660-Met 12923
• Endotoxin: Less than 1.0 EU per μg by the LAL method.
• Purity: >95% as determined by SDS-PAGE.
• Storage: For long term storage, the product should be stored at lyophilized state at-20°C or lower.Please avoid repeated freeze-thaw cycles. This product is stable after storage at:-20°C to-70°C for 12 months in lyophilized state;-70°C for 3 months under sterile conditions after reconstitution.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.

Gene Information

• Gene Name: MUC16 mucin 16, cell surface associated [ Homo sapiens ]
• Official Symbol: MUC16
• Synonyms: MUC16; mucin 16, cell surface associated; mucin-16; CA125; FLJ14303; CA-125; MUC-16; CA125 ovarian cancer antigen; ovarian carcinoma antigen CA125; ovarian cancer-related tumor marker CA125
• Gene ID: 94025
• mRNA Refseq: NM_024690
• Protein Refseq: NP_078966
• MIM: 606154
• UniProt ID: Q8WXI7

Reviews

07/28/2020

Critical for decision-making. Supports our research endeavors.

05/24/2018

Expert service quality. Valuable for research integrity.

06/05/2017

Dependable partner in experiments. Consistent and trustworthy.

Q&As

How does MUC16 interact with other components of the epithelial barrier and immune system?

MUC16 works with other mucins and immune components to maintain epithelial barrier function and immune responses.

How does MUC16 contribute to the formation of the mucosal barrier?

It aids in forming the mucosal barrier, protecting against pathogens and mechanical damage.

What potential therapeutic applications arise from targeting MUC16 in cancer treatment?

Targeting MUC16 in cancer therapy, especially in ovarian cancer, could be effective in inhibiting tumor growth and spread.

What is the impact of altered MUC16 activity on inflammatory and infectious diseases?

Altered MUC16 activity can impact the body's defense against inflammation and infection.

What is the primary role of MUC16 in epithelial cell function?

MUC16, a mucin protein, is essential for maintaining the protective mucous layer on epithelial surfaces.

What role does MUC16 play in cancer, particularly ovarian cancer?

MUC16 is associated with cancer progression, particularly in ovarian cancer, where it may facilitate tumor growth and metastasis.

How do genetic variations in MUC16 affect its function in mucosal protection and cancer development?

Genetic variations in MUC16 can lead to differences in mucosal integrity and potentially influence cancer susceptibility.